What laboratory tests indicate menopause in women, specifically assessing Follicle-Stimulating Hormone (FSH) levels and Estradiol (E2) levels?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: May 3, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

To assess menopause status, the most reliable laboratory tests are follicle-stimulating hormone (FSH) and estradiol levels, with FSH levels above the postmenopausal range and estradiol levels in the postmenopausal range suggesting menopause. When evaluating menopause status, it is essential to consider the clinical context, including age, menstrual history, and symptoms. According to the most recent guidelines 1, reasonable criteria for determining menopause include prior bilateral oophorectomy, age ≥60 years, or age <60 years and amenorrheic for 12 or more months in the absence of chemotherapy, tamoxifen, toremifene, or ovarian suppression, with FSH and estradiol levels in the postmenopausal range.

Key Laboratory Tests

  • FSH level: elevated levels above the postmenopausal range suggest menopause
  • Estradiol level: levels in the postmenopausal range support menopausal status

Considerations

  • Age: ≥60 years is considered postmenopausal
  • Menstrual history: amenorrhea for 12 or more months in the absence of chemotherapy, tamoxifen, toremifene, or ovarian suppression
  • Symptoms: menopausal symptoms such as hot flashes, night sweats, and vaginal dryness

Special Considerations

  • Women receiving an LHRH agonist or antagonist: menopausal status cannot be assigned
  • Women premenopausal at the beginning of adjuvant chemotherapy: amenorrhea is not a reliable indicator of menopausal status, and serial measurement of FSH and/or estradiol may be necessary to ensure postmenopausal status.

From the Research

Menopause Status Assessment

To assess menopause status in women, several laboratory tests can be used, including:

  • Follicle-stimulating hormone (FSH) levels: FSH levels are often used to indicate menopause status, with levels >40 IU/L typically indicating postmenopause 2. However, FSH levels can fluctuate and may not always be a reliable indicator of menopause status 3, 4, 5.
  • Oestradiol (E2) levels: E2 levels may also be used to assess menopause status, although the relationship between E2 and menopause is not always clear 3, 4.
  • Luteinizing hormone (LH) levels: LH levels may also be used to assess menopause status, particularly in conjunction with FSH levels 6, 5.
  • Vaginal pH: Vaginal pH has been suggested as a potential marker for menopause, with a pH >4.5 indicating postmenopause 2.

Limitations of FSH in Assessing Menopause Status

While FSH levels are often used to assess menopause status, there are several limitations to this approach, including:

  • FSH levels can fluctuate and may not always be a reliable indicator of menopause status 3, 4, 5.
  • There is considerable overlap in FSH levels between different reproductive stages, making it difficult to determine menopause status based on FSH alone 5.
  • Age and reproductive stage are the most important determinants of FSH levels, rather than menopause status itself 5.

Alternative Markers for Menopause

Alternative markers for menopause, such as vaginal pH, may be useful in conjunction with FSH and other hormone levels to assess menopause status 2. However, more research is needed to fully understand the relationship between these markers and menopause status.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Vaginal pH: A marker for menopause.

Journal of mid-life health, 2014

Research

The menopause: when it is all over or is it?

The Australian & New Zealand journal of obstetrics & gynaecology, 1994

Research

Estrogen replacement therapy in postmenopausal women: a study of the efficacy of estriol and changes in plasma gonadotropin levels.

Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, 2001

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.