Should levothyroxine (T4) be taken on the day of thyroid function test level drawn?

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Last updated: May 5, 2025 • View editorial policy

From the Research

No, levothyroxine should not be taken on the morning of thyroid lab tests, as this can cause a temporary spike in thyroid hormone levels, potentially leading to inaccurate test results and inappropriate dose adjustments. This recommendation is based on the study by 1, which found that thyroid hormone levels are affected by the time of blood sampling in patients taking levothyroxine. The study showed that serum levels of thyroid hormones can remain elevated for up to 9 hours after taking levothyroxine, which can lead to inaccurate test results.

To ensure accurate monitoring of thyroid function, patients should skip their morning dose of levothyroxine until after blood has been drawn for thyroid function tests, which measure TSH and free T4 levels. However, patients should resume taking their levothyroxine immediately after the blood test is completed, and they should not skip doses on any other days. For accurate monitoring, thyroid labs should ideally be drawn at consistent times, usually in the morning, and patients should maintain a consistent schedule of taking levothyroxine (typically 30-60 minutes before breakfast) on all other days to ensure stable blood levels for proper assessment of thyroid function.

It's also important to note that the management of hypothyroidism and the use of levothyroxine therapy should be based on clinical and laboratory findings, as recommended by 2. The study by 3 highlights the importance of precise and effective dose titration of levothyroxine to achieve optimal treatment outcomes, and the study by 4 demonstrates the value of pharmacist-mediated monitoring programs in improving TSH test compliance and dose adjustment outcomes in patients taking levothyroxine. However, the key takeaway is that levothyroxine should not be taken on the morning of thyroid lab tests to ensure accurate test results and optimal treatment outcomes.

Some key points to consider when managing patients on levothyroxine include:

  • Maintaining a consistent schedule of taking levothyroxine to ensure stable blood levels
  • Drawing thyroid labs at consistent times, usually in the morning
  • Skipping the morning dose of levothyroxine on the day of thyroid lab tests
  • Resuming levothyroxine immediately after the blood test is completed
  • Monitoring TSH and free T4 levels regularly to adjust the levothyroxine dose as needed.

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