What is the best laboratory test to monitor hypothyroidism (underactive thyroid) in pregnancy?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: February 18, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

Monitoring Hypothyroidism in Pregnancy

The best laboratory test to monitor hypothyroidism in pregnancy is Thyroid-Stimulating Hormone (TSH) levels, as it is crucial for assessing the thyroid function and adjusting the levothyroxine dose accordingly 1.

  • TSH levels should be monitored every 6 to 8 weeks during pregnancy to ensure that the serum TSH concentration is within the reference range 1.
  • Additionally, Free Thyroxine (FT4) levels should also be monitored to assess the effectiveness of levothyroxine therapy 1.
  • The target range for TSH levels may vary depending on the trimester, but generally, it is recommended to maintain a TSH level between 0.1-2.5 mU/L during the first trimester, 0.2-3.0 mU/L during the second trimester, and 0.3-3.5 mU/L during the third trimester.
  • It is essential to adjust the levothyroxine dose as needed to maintain target TSH and FT4 levels, with a typical starting dose of 50-100 mcg/day 1.
  • Regular monitoring of TSH and FT4 levels is crucial to prevent potential complications, such as fetal wastage or neuropsychological complications in the offspring due to thyroid insufficiency 1.

From the FDA Drug Label

For pregnant patients with pre-existing hypothyroidism, measure serum TSH and free-T4 as soon as pregnancy is confirmed and, at minimum, during each trimester of pregnancy. In pregnant patients with primary hypothyroidism, maintain serum TSH in the trimester-specific reference range The best laboratory test to monitor hypothyroidism in pregnancy is serum TSH and free-T4, with the goal of maintaining serum TSH in the trimester-specific reference range 2.

  • Key laboratory tests:
    • Serum TSH
    • Free-T4
  • Monitoring frequency:
    • As soon as pregnancy is confirmed
    • At minimum, during each trimester of pregnancy
    • Every 4 weeks until a stable dose is reached and serum TSH is within normal trimester-specific range

From the Research

Laboratory Tests for Monitoring Hypothyroidism in Pregnancy

The best laboratory test to monitor hypothyroidism in pregnancy is the serum thyroid-stimulating hormone (TSH) concentration, as it is the most sensitive indicator of thyroid function 3, 4, 5, 6, 7.

Interpretation of TSH Values

  • TSH values should be interpreted with regard to gestational age, as the reference ranges for TSH vary during different trimesters of pregnancy 5, 6.
  • The upper limit of the reference range for TSH in the first trimester is generally considered to be 2.5 mIU/L, while in the second and third trimesters, it is 3 mIU/L 3.
  • However, some studies suggest that the upper limit of the reference range for TSH may be higher, up to 4.0 mIU/L, especially in the second and third trimesters 6.

Trimester-Specific Reference Ranges

  • The reference ranges for TSH, tri-iodothyronine (T3), and thyroxine (T4) vary during different trimesters of pregnancy 7.
  • In the first trimester, the mean T3 and T4 values are lower, while the mean TSH value is higher compared to non-pregnant women 7.
  • The reference ranges for T3, T4, and TSH in the second and third trimesters are also different from those in non-pregnant women 7.

Monitoring and Treatment

  • Women with overt hypothyroidism should be treated promptly with levothyroxine to achieve serum TSH concentrations within the reference ranges for pregnancy as soon as possible 3, 5.
  • Women with subclinical hypothyroidism may also benefit from treatment with levothyroxine, especially if the TSH level is >10 mIU/L or thyroperoxidase antibodies are present 5.
  • Close monitoring of serum TSH levels is necessary to avoid overtreatment or undertreatment in pregnant women with hypothyroidism 4, 5.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Hypothyroidism in pregnancy.

The lancet. Diabetes & endocrinology, 2013

Research

Management of Hypothyroidism and Hypothyroxinemia During Pregnancy.

Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists, 2022

Research

Thyroid-Stimulating Hormone Values in Pregnancy: Cutoff Controversy Continues?

Journal of obstetrics and gynaecology of India, 2019

Research

Thyroid function tests in pregnancy.

Indian journal of medical sciences, 2003

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.