What does a positive lactoferrin test suggest?

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Last updated: May 6, 2025View editorial policy

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From the Guidelines

Positive lactoferrin suggests active intestinal inflammation, most commonly associated with inflammatory bowel disease (IBD) such as Crohn's disease or ulcerative colitis, with a sensitivity of 83% and specificity of 75% for detecting endoscopic inflammation 1. This finding is clinically significant as lactoferrin is a protein released by neutrophils during inflammation, making it a reliable biomarker for distinguishing inflammatory from non-inflammatory gastrointestinal conditions. When lactoferrin is detected in stool samples, it indicates neutrophil presence in the intestinal mucosa, which doesn't occur in functional disorders like irritable bowel syndrome (IBS). Some key points to consider include:

  • The diagnostic performance of fecal lactoferrin for detecting moderate to severe endoscopic inflammation has been reported in several studies, with a commercial assay cutoff of 7.25 mg/g 1.
  • Positive results should prompt further diagnostic evaluation, including colonoscopy with biopsies, imaging studies, and other inflammatory markers like C-reactive protein and erythrocyte sedimentation rate.
  • The level of lactoferrin can also help monitor disease activity and treatment response in patients with established IBD.
  • However, false positives can occur with infections, certain medications, or bleeding, so clinical correlation is essential for proper interpretation.
  • A threshold value in the range of 4.0–7.25 mg/g for fecal lactoferrin is recommended to optimize sensitivity for IBD, although this may not be the only factor to consider in clinical practice 1.

From the Research

Positive Lactoferrin Test Results

A positive lactoferrin test suggests the presence of an inflammatory condition in the colon, such as:

  • Ulcerative colitis
  • Crohn's disease
  • Ischemic colitis
  • Microscopic colitis
  • Inflammatory bowel disease (IBD) 2, 3

Diagnostic Accuracy

The sensitivity, specificity, and positive and negative predictive values of the fecal lactoferrin test for ulcerative or Crohn's colitis are:

  • Sensitivity: 90%
  • Specificity: 98%
  • Positive predictive value: 82%
  • Negative predictive value: 99% 2

Clinical Significance

Elevated lactoferrin levels are associated with:

  • Inflammatory disease activity
  • Mucosal healing and histologic improvement
  • Postoperative recurrence risk
  • Pouchitis prediction 3
  • Correlation with markers of inflammation, such as CRP, and stool frequency 4

Immunomodulatory and Antimicrobial Role

Lactoferrin has been shown to have:

  • Immunomodulatory and anti-inflammatory activities
  • Antimicrobial properties against bacteria and viruses, including SARS-CoV-2 5

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Fecal markers: calprotectin and lactoferrin.

Gastroenterology clinics of North America, 2012

Research

Relationship between faecal metronidazole and lactoferrin concentrations to clinical response of patients with Clostridioides difficile.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2020

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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