What is 0.25 milliliters (mL) in units on a 1 milliliter (mL) insulin syringe?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: May 8, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the FDA Drug Label

PRINCIPAL DISPLAY PANEL Levemir® Insulin detemir (rDNA origin) injection ... 100 units/mL (U-100) To determine the number of units in 0.25 mL of insulin, we need to calculate 25% of 100 units, since 0.25 mL is one-quarter of 1 mL.

  • 100 units/mL * 0.25 mL = 25 units 1

From the Research

In a 1 mL insulin syringe, 0.25 mL equals 25 units, as standard insulin syringes are calibrated so that 1 mL equals 100 units, making each 0.01 mL equivalent to 1 unit. This conversion is based on the standardization of U-100 insulin, which is the most common concentration used in the United States and many other countries 2. The use of unit markings on insulin syringes is crucial for accurate dosing, rather than estimating milliliters, as the syringes are specifically designed with unit graduations for precise measurement.

Some studies have investigated the accuracy and precision of insulin administration using various devices, including syringes and pens 3, 4. However, these studies do not affect the basic conversion of milliliters to units in a standard insulin syringe. Another study discussed the use of insulin syringes to reduce dosing errors for enoxaparin, a low molecular weight heparin, by administering whole milligram doses via an insulin syringe 5. Nevertheless, this does not change the fundamental conversion of 0.25 mL to units in a 1 mL insulin syringe.

The key point to remember is that 0.25 mL in a 1 mL insulin syringe corresponds to 25 units, based on the standard calibration of these syringes. This is essential for accurate insulin dosing and applies to the most commonly used concentration of insulin, U-100.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.