What are neurofilament antibodies (NFs)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: May 9, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

Neurofilament light chain (NfL) is a promising biomarker for neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), and its levels in cerebrospinal fluid and blood indicate axonal damage and neurodegeneration. Testing for NfL can help with earlier diagnosis, monitoring disease progression, and potentially evaluating treatment effectiveness 1. Patients with neurodegenerative diseases typically show significantly higher NfL levels compared to healthy individuals or those with non-neurodegenerative conditions. The advantage of NfL testing is that it can now be measured in blood samples using highly sensitive assays, making it less invasive than lumbar punctures. While NfL is not specific to one disease alone, when combined with clinical assessment and other diagnostic tools, it enhances diagnostic accuracy. For patients with neurodegenerative diseases, regular NfL monitoring may help track disease progression and potentially adjust care plans accordingly. The biological basis for NfL elevation is the breakdown of neuronal cytoskeleton during the degenerative process, releasing these proteins into the extracellular space and eventually into circulation 1.

Some key points to consider:

  • NfL levels are elevated in various neurodegenerative diseases, including ALS, frontotemporal dementia, and Alzheimer's disease 1.
  • NfL can be measured in both cerebrospinal fluid and blood, with blood sampling being less invasive 1.
  • The sensitivity and specificity of NfL as a biomarker can vary depending on the disease and population being studied 1.
  • NfL levels can be influenced by factors such as age, renal function, and body mass index, which should be considered when interpreting results 1.
  • Further research is needed to fully understand the role of NfL in neurodegenerative diseases and to establish its clinical utility 1.

In terms of clinical practice, NfL testing can be a valuable tool for diagnosing and monitoring neurodegenerative diseases, but it should be used in conjunction with other diagnostic tools and clinical assessment. Regular NfL monitoring can help track disease progression and adjust care plans accordingly. However, it is essential to consider the limitations and potential biases of NfL testing, as well as the need for further research to fully understand its clinical utility.

From the Research

Neurofilament Light Chain as a Biomarker

  • Neurofilament light chain (NfL) has emerged as a potential biomarker for amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) 2.
  • NfL is a structural component of the axonal cytoskeleton and is released into the cerebrospinal fluid (CSF) and blood as a consequence of axonal damage or degeneration 2, 3.
  • Elevated levels of NfL in CSF and blood have been associated with neurodegenerative diseases, including ALS, FTD, and Alzheimer's disease 2, 3, 4.

Clinical Applications of NfL

  • NfL has been shown to be a useful biomarker for diagnosing and monitoring ALS and FTD, with elevated levels correlating with disease progression and survival 2, 5.
  • NfL may also serve as a susceptibility/risk biomarker in populations at elevated risk for ALS, and as a prognostic biomarker when measured early in the course of established disease 5.
  • The use of NfL as a pharmacodynamic biomarker for therapeutic trials in ALS has also been proposed, with reductions in NfL levels potentially indicating a positive response to treatment 5.

Measurement and Interpretation of NfL

  • NfL can be quantified in CSF and blood, with blood levels being present at much lower concentrations compared to CSF 2, 6.
  • The interpretation of NfL levels may benefit from considering neurological symptoms and their relationship to blood NfL concentrations 4.
  • Longitudinal studies have shown that NfL levels can accumulate over time in response to neuronal injury, making it a potentially useful biomarker for monitoring disease progression and treatment efficacy 6.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.