What test helps differentiate the cause of Acute Kidney Injury (AKI)?

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Last updated: May 11, 2025View editorial policy

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From the Guidelines

The fractional excretion of sodium (FENa) test is the most helpful test to differentiate the cause of acute kidney injury (AKI). This test measures the percentage of sodium filtered by the kidneys that is excreted in the urine, helping distinguish between prerenal, intrinsic, and postrenal causes of AKI. A FENa less than 1% typically indicates prerenal AKI (caused by decreased blood flow to the kidneys), while a value greater than 2% suggests intrinsic renal damage 1. To perform this test, both urine and serum samples are collected to measure sodium and creatinine levels. Other useful tests include urine microscopy (to look for casts, cells, or crystals), urine osmolality, and the BUN-to-creatinine ratio.

In patients taking diuretics, the fractional excretion of urea (FEUrea) may be more reliable as diuretics can artificially elevate the FENa 1. These tests are valuable because they provide immediate information to guide treatment decisions before more definitive tests like kidney biopsy can be performed, allowing clinicians to quickly determine if the kidney injury is due to volume depletion, intrinsic kidney damage, or urinary tract obstruction. The use of biomarkers, such as neutrophil gelatinase–associated lipocalin (NGAL) and cystatin C, can also help improve the diagnostic accuracy of AKI and recognize the different pathophysiological processes 1.

Some key points to consider when interpreting the results of these tests include:

  • The clinical context in which the test is being performed
  • The presence of any underlying kidney disease or other comorbidities
  • The potential for false positives or false negatives
  • The need for prompt treatment and follow-up to prevent further kidney damage.

Overall, the FENa test, in combination with other diagnostic tests and clinical information, can help clinicians quickly and accurately diagnose the cause of AKI and guide treatment decisions to improve patient outcomes.

From the Research

Diagnostic Tests for Acute Kidney Injury

The following tests help differentiate the cause of acute kidney injury:

  • Urinalysis with microscopy to narrow the differential diagnosis 2
  • Measurement of serum creatinine level to assess the increase in serum creatinine 3, 2, 4, 5, 6
  • Fractional excretion of sodium to classify acute kidney injury as prerenal, intrinsic renal, or postrenal 5
  • Ultrasonography of the kidneys to rule out obstruction, particularly in older men 5, 6
  • Serum and urine electrolyte measurements to determine the underlying cause of acute kidney injury 6
  • Complete blood count to identify systemic illnesses that might cause poor renal perfusion or directly impair renal function 5

Laboratory Evaluation

The initial laboratory evaluation for acute kidney injury includes:

  • Measurement of serum creatinine level 3, 2, 4, 5, 6
  • Complete blood count 5
  • Urinalysis with microscopy 2
  • Fractional excretion of sodium 5
  • Serum and urine electrolyte measurements 6

Imaging Studies

Imaging studies, such as ultrasonography of the kidneys, are used to:

  • Rule out obstruction, particularly in older men 5, 6
  • Assess kidney size and structure 5

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Kidney Disease: Acute Kidney Injury.

FP essentials, 2021

Research

Acute Kidney Injury.

Primary care, 2020

Research

Acute kidney injury: a guide to diagnosis and management.

American family physician, 2012

Research

Acute Kidney Injury: Diagnosis and Management.

American family physician, 2019

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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