What testing is required to confirm Ehlers-Danlos Syndrome (EDS)?

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Last updated: May 20, 2025View editorial policy

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From the Guidelines

Confirming Ehlers-Danlos syndrome (EDS) requires a combination of clinical evaluation, genetic testing, and specialized assessments based on the suspected EDS subtype. A comprehensive diagnostic approach typically begins with a detailed medical history and physical examination to identify characteristic symptoms like joint hypermobility, skin hyperextensibility, and tissue fragility. Genetic testing is essential for most EDS subtypes, particularly for hypermobile EDS (hEDS), classical EDS, vascular EDS, and rarer forms. These tests look for specific gene mutations associated with each subtype, such as COL5A1 and COL5A2 for classical EDS or COL3A1 for vascular EDS. For hypermobile EDS, which lacks a specific genetic marker, diagnosis relies on clinical criteria including the Beighton score to assess joint hypermobility (a score of ≥5 out of 9 indicates hypermobility).

Some key points to consider in the diagnosis of EDS include:

  • A detailed medical history to identify symptoms and family history of the condition
  • Physical examination to assess joint hypermobility, skin hyperextensibility, and tissue fragility
  • Genetic testing for specific gene mutations associated with each EDS subtype
  • Use of clinical criteria such as the Beighton score for hypermobile EDS
  • Additional tests like skin biopsies and cardiovascular imaging may be necessary for certain subtypes. Although the study by 1 provides information on the diagnosis and management of aortic dissection, it does not directly address the diagnostic approach for EDS, highlighting the need to consider a broader range of evidence in forming a diagnostic strategy.

Given the complexity and variability of EDS, a multidisciplinary approach involving geneticists, cardiologists, and other specialists may be necessary to accurately diagnose and manage the condition, particularly in cases where vascular complications are a concern, as noted in the context of aortic dissection 1.

From the Research

Diagnostic Testing for EDS Syndrome

To confirm a diagnosis of EDS syndrome, several tests may be necessary. These include:

  • Gene panel testing with massively parallel sequencing, which is currently the gold standard for confirming diagnoses of the monogenic EDS types 2
  • A thorough clinical assessment, including medical history, family history, and physical examination, which remains important in the diagnostic process 2
  • Skin biopsy with electron microscopy, which can be helpful in deciding whether and when to perform genetic testing 3
  • Echocardiography, which may be useful in identifying cardiovascular complications, particularly in patients with vascular or classic EDS 4

Specific Testing for EDS Subtypes

The type of testing required may vary depending on the specific subtype of EDS suspected. For example:

  • For hypermobile EDS, routine cardiac evaluation and echocardiography are not required 4
  • For vascular EDS, echocardiography and cardiac evaluation are common, but may not always detect cardiovascular complications 4
  • For classic EDS, aortic dilation is common, and echocardiography may be useful in detecting this complication 4

Importance of Clinical Assessment

A thorough clinical assessment is essential in the diagnosis of EDS syndrome, as it can help guide management and surveillance, even in cases where a molecular diagnosis is not possible 2, 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Clinical diagnosis of the monogenic Ehlers-Danlos syndromes.

Medizinische Genetik : Mitteilungsblatt des Berufsverbandes Medizinische Genetik e.V, 2024

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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