What laboratory tests are considered for a neurological workup?

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From the Guidelines

A neurological workup should include brain imaging and cerebrospinal fluid (CSF) analysis as the initial steps, followed by other targeted tests based on clinical suspicion, as recommended by recent guidelines 1. When suspecting neurological disorders, a comprehensive workup is essential to confirm the diagnosis and rule out other possibilities.

Initial Steps

The workup typically starts with:

  • Brain imaging to evaluate structural abnormalities
  • Cerebrospinal fluid (CSF) analysis to assess for infections, autoimmune conditions, or other abnormalities, as suggested by the study on autoimmune encephalitis 1

Additional Tests

Other key laboratory tests may include:

  • Blood tests such as complete blood count (CBC), comprehensive metabolic panel, and thyroid function tests
  • Vitamin B12 and folate levels to assess for deficiencies that can cause neurological symptoms
  • Inflammatory markers like ESR and CRP to identify inflammatory conditions
  • Specific tests like autoimmune panels, heavy metal screening, and genetic testing, depending on the suspected diagnosis
  • Cerebrospinal fluid (CSF) analysis via lumbar puncture for evaluating infections, autoimmune conditions, and certain dementias

Specialized Tests

Additional specialized tests might be ordered based on clinical suspicion, including:

  • Paraneoplastic panels to screen for cancer-related neurological disorders
  • HIV testing when immunocompromise is suspected
  • Drug levels when medication toxicity is a concern The specific tests ordered depend on the patient's symptoms, medical history, and suspected diagnosis, with the neurologist tailoring the workup accordingly, as outlined in the proposed best practice recommendations for diagnosis and acute management of autoimmune encephalitis 1.

From the Research

Laboratory Tests for Neurological Workup

The following laboratory tests are considered for a neurological workup:

  • Blood cell count
  • Thyroid function tests
  • Renal function tests
  • Liver function tests
  • Blood glucose levels
  • HbA1c
  • Vitamin-B12
  • Immunofixation 2 These tests are recommended as basic screening tests for neuropathies and should be chosen according to the individual and family history, clinical presentation, and electrophysiological findings.

Additional Laboratory Investigations

Other laboratory investigations may be carried out if a specific phenotype is present or if unexpected changes of the disease course occur, such as:

  • Serologic testing for Lyme disease 3
  • Tests for hereditary neuropathies It is recommended to refer patients to a neuromuscular center if basic laboratory parameters fail to uncover the etiology 2.

Reference Laboratory Testing

Reference laboratory testing plays a critical role in the diagnosis and monitoring of patients with neurologic disorders, and an increasing number of essential but esoteric tests are performed at reference laboratories or other outside health care facilities 4.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Laboratory Tests for Neuropathies: What to do and to Avoid.

Journal of neuromuscular diseases, 2020

Research

Updated CDC Recommendation for Serologic Diagnosis of Lyme Disease.

MMWR. Morbidity and mortality weekly report, 2019

Research

Reference Laboratory Testing for Neurologic Disorders.

Clinics in laboratory medicine, 2020

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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