What is the initial workup for pheochromocytoma (adrenal gland tumor)?

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From the Guidelines

The initial workup for pheochromocytoma involves biochemical testing to detect excess catecholamines or their metabolites, with plasma free metanephrines being the best screening test, carrying a 99% sensitivity and an 89% specificity 1. The initial workup for pheochromocytoma is crucial in diagnosing this rare but potentially life-threatening condition.

  • Biochemical testing is the first step, which includes measuring circulating catecholamine metabolites such as normetanephrine and metanephrine.
  • The screening test of choice is plasma free metanephrines, which has a high sensitivity and specificity 1.
  • If biochemical testing is positive, imaging studies are then performed to localize the tumor, with CT scan with contrast of the abdomen and pelvis being the first imaging study, as most pheochromocytomas occur in the adrenal glands 1.
  • MRI is an alternative for patients who cannot receive contrast, and functional imaging with 123I-MIBG scintigraphy or PET scanning may be necessary for suspected extra-adrenal tumors or metastatic disease 1.
  • Genetic testing should also be considered, as approximately 40% of pheochromocytomas are associated with hereditary syndromes such as MEN2, von Hippel-Lindau disease, neurofibromatosis type 1, and SDH mutations 1. The workup should be conducted while monitoring blood pressure carefully, as these tumors can cause dangerous hypertensive episodes 1.
  • It is essential to consider the diagnosis of pheochromocytoma in anyone referred for resistant hypertension, given the high morbidity and mortality of not treating these tumors 1.
  • The recent Endocrine Society guideline recommends starting with computed tomography, with magnetic resonance imaging as an alternative, and metaiodobenzylguanidine scanning to further evaluate suspected metastatic disease 1.

From the Research

Initial Workup for Pheochromocytoma

The initial workup for pheochromocytoma involves biochemical testing to establish the diagnosis, followed by radiological imaging to localize the tumor. The key steps in the initial workup are:

  • Biochemical testing: measuring plasma free metanephrines and urinary fractionated metanephrines 2, 3, 4, 5
  • Radiological imaging: computed tomography (CT) or magnetic resonance imaging (MRI) to localize the tumor 2, 3

Biochemical Testing

Biochemical testing is the first step in diagnosing pheochromocytoma. The tests used include:

  • Plasma free metanephrines: this test is highly sensitive and specific for pheochromocytoma 2, 5
  • Urinary fractionated metanephrines: this test is also highly sensitive and specific for pheochromocytoma 2, 3, 4
  • Urinary free metanephrines: this test has been shown to be a reliable method for diagnosing pheochromocytoma, especially in Asian populations 4

Radiological Imaging

Once the biochemical diagnosis is established, radiological imaging is used to localize the tumor. The imaging tests used include:

  • Computed tomography (CT): this test is highly sensitive for localizing pheochromocytoma 2, 3
  • Magnetic resonance imaging (MRI): this test is also highly sensitive for localizing pheochromocytoma 2, 3
  • Scintigraphy with (123)I-metaiodobenzylguanidine (MIBG): this test is highly specific for pheochromocytoma, but less sensitive than CT or MRI 2, 3

Additional Testing

Additional testing may be necessary in some cases, including:

  • Provocative tests (glucagon) and suppression tests (clonidine): these tests may be necessary when baseline measurements are inconclusive 3
  • Positron emission tomography (PET) with (18)F-F-fluorodopamine (F-DA): this test is useful in metastatic disease 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Pheochromocytoma: diagnostic and therapeutic update.

Endocrinologia y nutricion : organo de la Sociedad Espanola de Endocrinologia y Nutricion, 2008

Research

Pheochromocytoma: current perspectives in the pathogenesis, diagnosis, and management.

Arquivos brasileiros de endocrinologia e metabologia, 2004

Research

Urinary Free Metanephrines for Diagnosis of Pheochromocytoma and Paraganglioma.

Endocrinology and metabolism (Seoul, Korea), 2021

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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