What is the use of Methylene Blue (methylthioninium chloride)?

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Last updated: May 25, 2025View editorial policy

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From the Guidelines

Methylene blue is the primary treatment for methemoglobinemia, and it should be administered at a dose of 1-2 mg/kg intravenously, with the possibility of repeat dosing at 1 mg/kg if methemoglobinemia does not significantly decrease within 30-60 minutes 1. The medication works by acting as an electron donor, converting methemoglobin back to hemoglobin in cases of methemoglobinemia. Some key points to consider when using methylene blue include:

  • It should be used with caution in patients with G6PD deficiency, as it may trigger hemolytic anemia 1.
  • It is contraindicated in patients taking certain psychiatric medications, particularly MAO inhibitors and SSRIs, due to the risk of serotonin syndrome 1.
  • It may cause temporary blue-green discoloration of urine and skin.
  • The risk of worsening of methemoglobinemia increases with repeated doses, with toxic levels of MB reached at a total dose > 7 mg/kg 1.
  • In cases of worsening methemoglobinemia after MB treatment, urgent exchange transfusion should be performed 1.
  • Methylene blue should be used cautiously in patients with renal failure and in anesthetized patients where it may inhibit guanylate cyclase, decreasing nitric oxide-mediated vasodilatation leading to systemic and pulmonary hypertension 1. Other treatment modalities that have been described for methemoglobinemia include exchange transfusion, hyperbaric oxygen therapy, and ascorbic acid, but methylene blue remains the most widely accepted and effective treatment 1.

From the FDA Drug Label

The FDA drug label does not answer the question.

From the Research

Methylene Blue Uses

  • Methylene blue is used in the treatment of methemoglobinemia at a dose of 1 to 2 mg/kg intravenously 2
  • It is also used as a diagnostic substance during some diagnostic procedures and as a part of the treatment of several diseases including methemoglobinemia, vasoplegic syndrome, ifosfamide-induced encephalopathy, and cyanide intoxication 3
  • Methylene blue has been used as a prophylactic agent to prevent ifosfamide-induced encephalopathy 4, 5

Methylene Blue and Ifosfamide-Induced Encephalopathy

  • Ifosfamide-induced encephalopathy is a rare and serious adverse reaction that can occur in patients receiving ifosfamide infusion 6
  • Methylene blue has been used as a treatment for ifosfamide-induced encephalopathy, with some studies showing favorable responses 4, 5, 6
  • The use of methylene blue in the treatment of ifosfamide-induced encephalopathy is controversial, with some studies suggesting that it may be effective while others raise concerns about its safety and efficacy 3, 6

Safety and Efficacy of Methylene Blue

  • Methylene blue can cause dose-dependent toxic effects, including hemolysis, methemoglobinemia, nausea, vomiting, chest pain, dyspnea, and hypertension 3
  • The use of methylene blue has been associated with serotonergic syndrome, a potentially life-threatening condition 3, 6
  • More studies are needed to fully understand the safety and efficacy of methylene blue in the treatment of ifosfamide-induced encephalopathy and other conditions 3, 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Methylene blue.

American journal of therapeutics, 2003

Research

Hydration, methylene blue, and thiamine as a prevention regimen for ifosfamide-induced encephalopathy.

Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners, 2019

Research

Methylene blue and ifosfamide-induced encephalopathy: Myth or reality?

Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners, 2021

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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