What substrate from skeletal muscle supports hepatic (liver) gluconeogenesis?

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Last updated: May 25, 2025View editorial policy

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From the Research

Alanine is the primary substrate from skeletal muscle that supports hepatic gluconeogenesis, as evidenced by the most recent and highest quality study 1.

Key Points

  • During periods of fasting or exercise, skeletal muscle releases alanine into the bloodstream, which is then taken up by the liver.
  • In the liver, alanine is converted to pyruvate through a process called transamination, and this pyruvate serves as a precursor for glucose production via gluconeogenesis.
  • This metabolic pathway, known as the glucose-alanine cycle, is crucial for maintaining blood glucose levels during periods of energy demand.
  • Skeletal muscle produces alanine through the transamination of pyruvate, using amino groups from branched-chain amino acids.
  • Other amino acids like glutamine are also released from muscle and can contribute to gluconeogenesis, but alanine is quantitatively the most significant amino acid substrate in this inter-organ metabolic cooperation, as supported by studies 2, 1.

Mechanism of Action

  • The glucose-alanine cycle is a critical mechanism for maintaining glucose homeostasis, particularly during periods of fasting or exercise.
  • Alanine is produced in skeletal muscle through the transamination of pyruvate, and then transported to the liver where it is converted to glucose via gluconeogenesis.
  • This process not only provides substrate for hepatic glucose production but also serves as a mechanism for transporting nitrogen from muscle to the liver for eventual excretion as urea.

Clinical Implications

  • Understanding the role of alanine in hepatic gluconeogenesis has important implications for the management of glucose metabolism in various clinical contexts, such as diabetes and sepsis.
  • The maintenance of gluconeogenesis from glycerol, but not from alanine, lactate, and pyruvate, in liver perfusion of rats with early and late sepsis, highlights the potential importance of glycerol as a precursor for glucose production in certain clinical scenarios 3.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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