What is the optimal dosage and type of Nicotinamide adenine dinucleotide (NAD+) to rejuvenate healthy cells?

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Last updated: May 29, 2025View editorial policy

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From the Guidelines

To rejuvenate healthy cells, a daily NAD+ supplement dose of 250-500mg is generally recommended, with Nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN) being the best types of NAD+ precursors due to their superior bioavailability. The importance of maintaining cellular NAD+ levels is highlighted in a study by Aytekin et al. 1, which discusses the role of NAD+ in energy metabolism and its decline with age. This study suggests that supplementation with vitamin B3, a precursor to NAD+, can help prevent cellular energy crisis and mitochondrial dysfunction.

When considering the best type of NAD+ precursor, it is essential to look at the bioavailability and effectiveness of different forms. NMN and NR are considered superior to direct NAD+ supplementation due to their ability to boost cellular NAD+ levels effectively. A study on Wistar rats demonstrated that supplementation with high doses of vitamin B3 (≥500 mg/kg) can prevent the decline of cellular NAD+ levels and associated cellular damage 1.

Some key points to consider when taking NAD+ supplements include:

  • Taking the supplement in the morning with food to maximize absorption and minimize potential side effects
  • Starting with a lower dose (250mg) and gradually increasing if needed
  • Combining supplementation with healthy lifestyle practices like regular exercise, quality sleep, and a nutritious diet
  • Monitoring individual responses and adjusting the supplement regimen accordingly

It is crucial to note that while supplementation can help maintain cellular function, it is not a replacement for a healthy lifestyle. By combining NAD+ supplementation with healthy habits, individuals can support their overall health and well-being. As highlighted in the study by Aytekin et al. 1, maintaining cellular NAD+ levels is essential for energy production, DNA repair, and activating sirtuins, which regulate cellular health and longevity.

From the FDA Drug Label

DOSAGE & ADMINISTRATION Usual Adult dose is one or two tablets taken once or twice a day with or without food or as prescribed by a physician. The FDA drug label does not answer the question.

From the Research

NAD+ Rejuvenation

To rejuvenate healthy cells, increasing NAD+ levels is a promising therapeutic strategy. The best type of NAD+ precursor is still being researched, but several options have shown potential benefits:

  • Nicotinamide riboside (NR) 2, 3, 4
  • Nicotinamide mononucleotide (NMN) 5, 6, 4
  • Nicotinamide (NAM) 5
  • Nicotinic acid (NA) 5, 2

Dosage and Efficacy

The optimal dosage of NAD+ precursors is not yet established, and more research is needed to determine the most effective dosing regimens 4. However, studies have shown that chronic supplementation with NR and NMN can increase NAD+ levels and improve physiological function in healthy middle-aged and older adults 3, 4.

Safety and Tolerability

NAD+ precursors have been found to be safe and well-tolerated in human clinical trials 5, 3, 4. However, potential risks and side effects, such as the accumulation of toxic metabolites and tumorigenesis, need to be further investigated 5.

Food Sources

NAD+ precursors can be found in certain food sources, including:

  • Vegetables 6
  • Meat 6
  • Milk 6
  • Fermented beverages 6

Future Directions

Further research is needed to fully understand the potential benefits and risks of NAD+ precursors for human health 5, 6, 4. This includes investigating the effects of different dosing regimens, study durations, and population groups, as well as exploring new food sources and processing strategies to maintain and enhance the physiological benefits of NAD+ precursors 6, 4.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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