Is a single dose or a 3-dose regimen of benzathine penicillin G (Bicillin) recommended for routine treatment of acute syphilis?

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From the Guidelines

A single dose of benzathine penicillin G (Bicillin) is recommended for routine treatment of acute syphilis in its early stages (primary, secondary, and early latent syphilis of less than one year's duration). The standard regimen is a single intramuscular injection of 2.4 million units of benzathine penicillin G. For patients with late latent syphilis (infection of more than one year's duration) or syphilis of unknown duration, a three-dose regimen is recommended, consisting of 2.4 million units of benzathine penicillin G administered intramuscularly once weekly for three consecutive weeks (total 7.2 million units) 1. This difference in treatment duration is based on the understanding that longer-standing infections require more prolonged therapy to effectively eliminate the spirochete Treponema pallidum, which can establish persistent infection in tissues.

Some key points to consider:

  • Penicillin remains the treatment of choice for syphilis because T. pallidum is highly sensitive to penicillin, and no resistance has been documented despite decades of use 1.
  • For patients with penicillin allergy, doxycycline 100 mg orally twice daily for 14 days (early syphilis) or 28 days (late syphilis) may be used as an alternative, though desensitization to penicillin is preferred for pregnant women with penicillin allergy 1.
  • Data on 1 versus 3 doses of intramuscular benzathine penicillin G (BPG) for treatment of early syphilis in human immunodeficiency virus (HIV)–infected patients remain limited, though most studies suggest no difference in serologic outcomes between 1 and >1 dose of BPG 1.

Overall, the most recent and highest quality study 1 supports the use of a single dose of benzathine penicillin G for early syphilis, with a three-dose regimen recommended for late latent syphilis or syphilis of unknown duration.

From the FDA Drug Label

DOSAGE AND ADMINISTRATION ... Syphilis Primary, secondary, and latent—2,400,000 units (1 dose). Late (tertiary and neurosyphilis)—2,400,000 units at 7-day intervals for three doses.

The recommended regimen for routine treatment of acute syphilis is a single dose of 2,400,000 units of benzathine penicillin G (Bicillin) for primary, secondary, and latent syphilis 2. However, for late (tertiary and neurosyphilis) syphilis, a 3-dose regimen is recommended, with 2,400,000 units administered at 7-day intervals 2.

From the Research

Treatment Regimens for Acute Syphilis

  • A single dose of benzathine penicillin G (Bicillin) is recommended for the routine treatment of acute syphilis, as supported by studies 3, 4, 5, 6.
  • The use of a single dose of 2.4 million units of benzathine penicillin G has been shown to be effective in treating early syphilis, with treatment success rates of 90% to 100% 5.
  • Multiple-dose treatment of early syphilis is not necessarily more effective, especially in HIV-infected individuals, as evidenced by studies 3, 6.
  • The Centers for Disease Control and Prevention recommend a single dose of benzathine penicillin G for the treatment of early syphilis, including in HIV-infected patients 3, 4.

Comparison of Single-Dose and 3-Dose Regimens

  • A randomized clinical trial found that a 3-dose regimen of benzathine penicillin G did not improve syphilis serological outcomes compared to a single dose 3.
  • Another study found that treatment response was the same among those receiving 1 or >1 dose of benzathine penicillin G, with a treatment response rate of 92% 6.
  • The use of a single dose of benzathine penicillin G is supported by the majority of the evidence, with no significant benefit shown for multiple-dose regimens 3, 4, 5, 6.

Special Considerations

  • HIV-infected persons with syphilis may require closer monitoring and follow-up, as they may be at increased risk for asymptomatic neurosyphilis 4.
  • Pregnant women with syphilis should be treated with penicillin, as it is the most effective treatment for preventing congenital syphilis 5.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Single Dose Versus 3 Doses of Intramuscular Benzathine Penicillin for Early Syphilis in HIV: A Randomized Clinical Trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2017

Research

Management of adult syphilis.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2011

Research

A single dose of benzathine penicillin G is as effective as multiple doses of benzathine penicillin G for the treatment of HIV-infected persons with early syphilis.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2015

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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