From the Research
Yes, natural Glucagon-like peptide-1 (GLP-1) levels can be measured, but it presents several challenges. The measurement is typically done through specialized blood tests that use immunoassays or mass spectrometry techniques. These tests need to be performed in fasting conditions, as GLP-1 levels rise after meals. A major challenge in measuring natural GLP-1 is its very short half-life of only 1-2 minutes in circulation, as it is rapidly degraded by the enzyme dipeptidyl peptidase-4 (DPP-4) 1. For accurate measurement, blood samples must be collected with DPP-4 inhibitors and protease inhibitors to prevent this rapid degradation. Additionally, there are different forms of GLP-1 (active and total), and tests may measure either or both. The measurement is primarily done in research settings rather than routine clinical practice, as the results have limited direct clinical application for most patients. If GLP-1 measurement is needed, it's essential to use a laboratory experienced with these specialized assays and proper sample handling techniques, considering the variability in commercially available kits 2. Recent studies have focused on the development of GLP-1 receptor agonists for the treatment of type 2 diabetes, highlighting the importance of accurate GLP-1 measurement in clinical research 3, 4. Key considerations for measuring GLP-1 levels include:
- Using specialized blood tests with immunoassays or mass spectrometry techniques
- Performing tests in fasting conditions
- Collecting blood samples with DPP-4 inhibitors and protease inhibitors
- Selecting a laboratory with experience in GLP-1 measurement and proper sample handling techniques
- Being aware of the different forms of GLP-1 and the variability in commercially available kits.