What are the causes of low C-peptide (Connecting peptide) levels?

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Last updated: February 23, 2025View editorial policy

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From the Guidelines

Low C-peptide levels are primarily caused by conditions that impair insulin production in the pancreas, with the most common cause being type 1 diabetes, as evidenced by the most recent study 1 published in 2025. C-peptide is a byproduct of insulin production, so its levels directly reflect insulin synthesis. The most common cause of low C-peptide is type 1 diabetes, where autoimmune destruction of pancreatic beta cells leads to decreased insulin and C-peptide production. Other significant causes include advanced type 2 diabetes with beta cell burnout, pancreatitis, pancreatic cancer, and cystic fibrosis-related pancreatic insufficiency. Certain medications, such as exogenous insulin therapy, can also suppress endogenous insulin production and consequently lower C-peptide levels. Less common causes include rare genetic disorders affecting insulin synthesis, severe malnutrition, and Addison's disease. It's essential to note that low C-peptide levels should always be interpreted in the context of blood glucose levels and other clinical factors, as recommended by recent guidelines 1 and 1. In cases of unexplained low C-peptide, further investigation of pancreatic function and autoimmune markers may be necessary to determine the underlying cause and guide appropriate treatment, as suggested by recent studies 1 and guidelines 1 and 1. Key points to consider when evaluating low C-peptide levels include:

  • Clinical presentation and medical history
  • Blood glucose levels and other laboratory results
  • Presence of autoimmune markers or other underlying conditions
  • Potential impact of medications or other factors on insulin production. Recent studies 1, 1, and 1 provide valuable insights into the diagnosis and management of diabetes, highlighting the importance of considering C-peptide levels in the context of overall clinical presentation and laboratory results.

From the Research

Causes of Low C-Peptide Levels

  • Low C-peptide levels can be caused by the decline of pancreatic beta-cell function over time, especially in individuals with type 1 diabetes 2
  • The rate of decline in C-peptide levels is significantly related to the age of onset of type 1 diabetes, with earlier onset resulting in a faster decline 2
  • C-peptide levels can also be affected by the duration of the disease, with longer duration resulting in lower C-peptide levels 2, 3
  • Low C-peptide levels have been associated with poor metabolic control, as measured by HbA1c, and an increased risk of complications such as nephropathy, neuropathy, foot ulcers, and retinopathy 2
  • Severe hypoglycaemia is also associated with low C-peptide levels, suggesting that individuals with low C-peptide levels may be at a higher risk of hypoglycaemic episodes 2, 3

Factors Influencing C-Peptide Levels

  • Age of onset of type 1 diabetes: earlier onset is associated with a faster decline in C-peptide levels 2
  • Duration of disease: longer duration is associated with lower C-peptide levels 2, 3
  • Metabolic control: poor metabolic control, as measured by HbA1c, is associated with low C-peptide levels 2
  • Presence of complications: low C-peptide levels are associated with an increased risk of complications such as nephropathy, neuropathy, foot ulcers, and retinopathy 2
  • Hypoglycaemia: severe hypoglycaemia is associated with low C-peptide levels 2, 3

Measurement of C-Peptide Levels

  • C-peptide levels can be measured using various assays, including glucagon stimulation C-peptide testing, which is recommended due to its balance of sensitivity and practicality 4
  • Modern assays can detect low levels of C-peptide, which can be used to guide diabetes diagnosis and management 4
  • C-peptide levels can be associated with diabetes type and duration of disease, with a level of less than 0.2 nmol/l associated with a diagnosis of type 1 diabetes mellitus (T1DM) 4

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Low levels of C-peptide have clinical significance for established Type 1 diabetes.

Diabetic medicine : a journal of the British Diabetic Association, 2015

Research

A Practical Review of C-Peptide Testing in Diabetes.

Diabetes therapy : research, treatment and education of diabetes and related disorders, 2017

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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