What is the comb test and its significance in assessing hair loss?

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Last updated: June 12, 2025View editorial policy

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From the Guidelines

The comb test is a valuable diagnostic tool for assessing hair loss, allowing clinicians to differentiate between normal shedding and pathological conditions, and its results are significant in evaluating the severity of hair loss and monitoring treatment effectiveness. To perform the test, a patient refrains from shampooing for 24 hours, then combs their hair forward from the back to the front over a light-colored surface or sheet of paper for about 60 seconds. The shed hairs are counted, with normal shedding typically being 10 or fewer hairs, while counts exceeding this threshold may indicate active hair loss 1.

Key Points to Consider

  • The comb test provides an objective measure of hair loss, helping clinicians assess the severity of the condition and monitor progression over time.
  • The test is non-invasive, can be performed at home, and provides quantifiable data that complements other diagnostic methods like the pull test or trichoscopy.
  • Results should be interpreted alongside clinical history and examination, considering factors like hair length, texture, and recent grooming practices that can influence outcomes.
  • The comb test is significant in evaluating the effectiveness of treatments for hair loss, such as platelet-rich plasma (PRP) therapy, which has shown promise in increasing hair density and improving hair morphology in patients with androgenetic alopecia (AGA) 1.

Clinical Application

The comb test can be used in clinical practice to monitor the effectiveness of treatments for hair loss, such as PRP therapy, and to adjust treatment protocols as needed. By combining the comb test with other diagnostic methods and clinical evaluation, clinicians can provide personalized care and optimize treatment outcomes for patients with hair loss. Further research is needed to standardize the use of the comb test and to evaluate its effectiveness in conjunction with other treatments for hair loss, such as PRF (platelet-rich fibrin) therapy, which has also shown promise in treating AGA 1.

From the Research

Comb Test Description

  • The comb test is a method used to assess hair loss, although it is not explicitly mentioned in the provided studies.
  • However, similar methods such as the "pull test" or "hair pull test" are mentioned in the studies, which involve gently pulling on a group of hairs to assess the amount of shedding [ 2 ].

Results and Significance

  • The comb test, or similar methods, can help diagnose and monitor hair loss conditions such as telogen effluvium (TE) and androgenetic alopecia (AGA) [ 3, 4, 5 ].
  • The test can provide information on the amount of hair shedding, which can be useful in assessing the severity of hair loss [ 6, 2 ].
  • The results of the comb test can also be used to monitor the effectiveness of treatments for hair loss conditions [ 2 ].
  • It is essential to note that the comb test is not a definitive diagnostic tool and should be used in conjunction with other diagnostic methods, such as medical history, physical examination, and other tests like trichogram, phototrichogram, or scalp biopsy [ 3, 6, 4, 5 ].

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Telogen effluvium: a comprehensive review.

Clinical, cosmetic and investigational dermatology, 2019

Research

Telogen Effluvium - a review of the science and current obstacles.

Journal of dermatological science, 2021

Research

Hair evaluation methods: merits and demerits.

International journal of trichology, 2009

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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