What are the diagnostic methods for Leprosy (Hansen's disease)?

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Last updated: February 26, 2025 • View editorial policy

From the Research

Diagnosing leprosy (Hansen's disease) involves a combination of clinical and laboratory methods, including physical examination, skin slit smear test, skin biopsies, PCR testing, and serological tests, as highlighted in the most recent study 1. The diagnosis of leprosy typically begins with a thorough physical examination looking for characteristic skin lesions with reduced sensation, thickened peripheral nerves, and muscle weakness.

  • A skin slit smear test is essential, where material from skin lesions is examined microscopically for Mycobacterium leprae bacteria.
  • Skin biopsies provide definitive diagnosis by revealing histopathological changes and the presence of acid-fast bacilli.
  • PCR testing can detect M. leprae DNA in tissue samples, while serological tests may identify antibodies against the bacteria. Early diagnosis is crucial as prompt treatment with multidrug therapy (MDT) can prevent disability, as noted in 2 and 1. The standard MDT regimen includes dapsone, rifampicin, and clofazimine, with treatment duration ranging from 6-12 months for paucibacillary leprosy to 12-24 months for multibacillary forms, as discussed in 3 and 4. Regular follow-up is necessary to monitor treatment response and manage potential complications like nerve damage or reactions, as emphasized in 1 and 5. It is essential to note that while various treatment options are available, the most effective approach is often a combination of these methods, and the choice of treatment should be guided by the severity of the disease and the patient's overall health, as highlighted in 1.

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