What is the diagnostic workup for the cause of pulmonary embolism?

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From the Guidelines

The diagnostic workup for pulmonary embolism (PE) should begin with clinical assessment using validated tools like the Wells score or Geneva score to determine pretest probability, followed by D-dimer testing for patients with low or intermediate probability, and imaging studies such as CT pulmonary angiography (CTPA) for those with high pretest probability or elevated D-dimer, as recommended by the most recent guidelines 1. The clinical assessment is crucial in determining the pretest probability of PE, which guides the subsequent diagnostic steps. For patients with low or intermediate probability, D-dimer testing is a useful initial step; a negative D-dimer can effectively rule out PE in these groups 1. If D-dimer is elevated or if the patient has high pretest probability, imaging studies are necessary.

  • CT pulmonary angiography (CTPA) is the gold standard imaging test, offering excellent sensitivity and specificity for detecting emboli in the pulmonary arteries.
  • In patients with contraindications to CTPA (such as renal failure or contrast allergy), ventilation-perfusion (V/Q) scanning is an alternative 1.
  • Lower extremity compression ultrasonography should be performed to identify deep vein thrombosis (DVT), the most common source of PE. For patients with unexplained or recurrent PE, additional testing may include:
  • Thrombophilia screening (factor V Leiden, prothrombin gene mutation, protein C/S deficiency, antithrombin deficiency, antiphospholipid antibodies), particularly in younger patients or those with family history of thrombosis.
  • Cancer screening may be warranted in patients with unprovoked PE, especially those over 40, as PE can be the first manifestation of occult malignancy 1. Echocardiography is useful to assess right ventricular function and pulmonary pressures, which helps with risk stratification and management decisions 1. The diagnostic approach should be guided by the most recent and highest quality evidence, prioritizing patient outcomes in terms of morbidity, mortality, and quality of life 1.

From the Research

Diagnostic Workup for Pulmonary Embolism

The diagnostic workup for pulmonary embolism (PE) involves a combination of clinical assessment, laboratory tests, and imaging studies.

  • The initial step in the diagnostic workup is to assess the clinical probability of PE using a clinical decision rule, such as the Wells score or the Geneva score 2, 3, 4.
  • Patients with a low clinical probability and a normal D-dimer concentration (<500 ng/mL) are considered unlikely to have PE, and further diagnostic testing and anticoagulant therapy can be withheld 2.
  • For patients with a moderate or high clinical probability, or those with a low clinical probability and an elevated D-dimer level, further diagnostic testing is necessary 2, 3, 4.
  • Imaging studies, such as compression ultrasonography of the legs, multidetector computed tomography angiography, or ventilation-perfusion scanning, can be used to diagnose or exclude PE 3, 4, 5.
  • Pulmonary angiography may be necessary in some cases where the clinical suspicion for PE remains high, despite negative results from less invasive studies 4.

Laboratory Tests

  • D-dimer testing is a useful tool in the diagnostic workup of PE, as a negative result can help exclude the diagnosis in patients with a low clinical probability 2, 3, 4.
  • However, a positive D-dimer result is not specific for PE and can be elevated in a variety of other conditions 5.

Imaging Studies

  • Multidetector computed tomography angiography is the diagnostic test of choice for PE when available and appropriate for the patient 4.
  • Ventilation-perfusion scanning is an acceptable alternative to computed tomography angiography in select settings 4.
  • Pulmonary angiography is the gold standard for diagnosing PE, but it is invasive and usually reserved for cases where the clinical suspicion remains high despite negative results from less invasive studies 4.

Clinical Decision Tools

  • Clinical decision tools, such as the Wells score or the Geneva score, can help guide the diagnostic workup and reduce the number of unnecessary tests 2, 3, 4, 6.
  • The use of clinical decision tools can also help improve the yield of pulmonary CT angiography and reduce the risk of false positives 6.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Deep vein thrombosis and pulmonary embolism.

Lancet (London, England), 2016

Research

Diagnosis of pulmonary embolism.

CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne, 2003

Research

Role of Clinical Decision Tools in the Diagnosis of Pulmonary Embolism.

AJR. American journal of roentgenology, 2017

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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