What labs are used to evaluate Acute Kidney Injury (AKI)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: July 5, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

To evaluate Acute Kidney Injury (AKI), the primary laboratory tests include serum creatinine, blood urea nitrogen (BUN), and urine output measurement, which are essential for diagnosis, determining severity, and identifying the underlying cause. The diagnosis of AKI is based on an increase in creatinine by 0.3 mg/dL within 48 hours or an increase in serum creatinine to 1.5 times baseline within 7 days, or urine volume less than 0.5 mL/kg/hr for 6 hours 1.

Key Laboratory Tests for AKI Evaluation

  • Serum creatinine: a cornerstone measurement for detecting and staging AKI
  • Blood urea nitrogen (BUN): helps in assessing the severity of AKI
  • Urine output measurement: crucial for diagnosing AKI, with output less than 0.5 mL/kg/hour for 6 hours suggesting kidney injury
  • Urinalysis: to check for proteinuria, hematuria, and the presence of casts or crystals, aiding in determining the etiology
  • Urine sodium and fractional excretion of sodium (FENa): help differentiate between prerenal causes (FENa <1%) and intrinsic renal damage (FENa >2%)
  • Complete blood count: may reveal anemia or signs of hemolysis
  • Electrolyte panels: to identify imbalances like hyperkalemia or metabolic acidosis that often accompany AKI Additional tests such as kidney ultrasound, complement levels, antinuclear antibodies, or ANCA testing may be necessary in specific cases to rule out obstruction or suspect glomerulonephritis 1.

Importance of Early Detection and Monitoring

Early detection and monitoring of AKI are critical, as AKI can lead to significant morbidity and mortality, and its incidence is rising worldwide 1. Monitoring patients with AKI involves measurements of serum creatinine and urine output to stage the severity of the condition 1. Patients at increased risk for AKI should be tested with measurements of serum creatinine and urine output to detect AKI, with the frequency and duration of monitoring individualized based on patient risk and clinical course 1.

From the Research

Labs Used to Evaluate Acute Kidney Injury (AKI)

The following labs are used to evaluate AKI:

  • Serum creatinine level 2, 3, 4
  • Complete blood count 2
  • Urinalysis 2, 5, 4, 6
  • Fractional excretion of sodium (FeNa) 2, 5
  • Fractional excretion of urea (FeU) 5
  • Ultrasonography of the kidneys 2, 3
  • Serum and urinary biomarkers such as cystatin C, neutrophil gelatinase-associated lipocalin, and uric acid 4
  • Urinary biochemistry, including measurement of sodium, chloride, phosphate, and strong ion difference (SIDu) 6
  • Blood physicochemical parameters, including phosphate, unmeasured anions (SIG), and albumin 6

Additional Tests

Other tests that may be used to evaluate AKI include:

  • Measurement of electrolyte imbalances 2
  • Assessment of acid-base balance 3, 6
  • Renal replacement therapy (dialysis) in severe cases 2, 3

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.