What investigations are needed to exclude malignancy in a patient with gradual onset of bilateral axillary acanthosis nigricans (AN) over 6 months, aside from checking Thyroid Function Tests (TFTs)?

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From the Guidelines

To exclude malignancy in a patient with bilateral axillary acanthosis nigricans, a comprehensive diagnostic workup including thorough physical examination, laboratory investigations, and imaging studies such as chest X-ray, abdominal and pelvic CT scan or MRI, and upper gastrointestinal endoscopy is necessary. The patient's gradual onset of symptoms over 6 months, absence of constitutional symptoms, and lack of other risk factors aside from smoking, necessitate a careful evaluation to rule out underlying malignancy. Initial laboratory investigations should include complete blood count, comprehensive metabolic panel, fasting glucose, HbA1c, and fasting insulin levels to assess for insulin resistance, as well as tumor markers appropriate for age and sex, including CEA, CA 19-9, CA-125 (in females), and PSA (in males) 1.

Given the patient's presentation, it is crucial to consider the possibility of gastric adenocarcinoma, which is commonly associated with malignant acanthosis nigricans. Therefore, upper gastrointestinal endoscopy is recommended to directly visualize and biopsy any suspicious lesions in the stomach or esophagus. Age-appropriate cancer screening should also be completed, including colonoscopy, mammography for females, and skin examination. In cases where initial investigations are inconclusive but clinical suspicion remains high, a PET-CT scan may help identify occult malignancies, as suggested by the ESMO clinical recommendations for diagnosis, treatment, and follow-up of cancers of unknown primary site 1.

The diagnostic workup should be guided by the principles outlined in the ESMO minimum clinical recommendations for diagnosis, treatment, and follow-up of cancers of unknown primary site, which emphasize the importance of thorough physical examination, basic blood and biochemistry survey, urinalysis, fecal occult blood test, and imaging studies such as chest X-ray and CT scan of the abdomen and pelvis 1. By prioritizing these investigations, clinicians can effectively exclude malignancy and provide appropriate management for patients with bilateral axillary acanthosis nigricans.

Key investigations to exclude malignancy in this patient include:

  • Thorough physical examination
  • Laboratory investigations: complete blood count, comprehensive metabolic panel, fasting glucose, HbA1c, fasting insulin levels, and tumor markers (CEA, CA 19-9, CA-125, PSA)
  • Imaging studies: chest X-ray, abdominal and pelvic CT scan or MRI
  • Upper gastrointestinal endoscopy
  • Age-appropriate cancer screening: colonoscopy, mammography, skin examination
  • PET-CT scan if initial investigations are inconclusive but clinical suspicion remains high.

From the Research

Investigations to Exclude Malignancy

To exclude malignancy in a patient with gradual onset of bilateral axillary acanthosis nigricans (AN) over 6 months, the following investigations are recommended:

  • Fasting lipoprotein profile
  • Fasting glucose
  • Fasting insulin
  • Hemoglobin
  • Alanine aminotransferase
  • Radiological investigations, such as:
    • Plain radiography
    • Ultrasonography
    • Magnetic resonance imaging (MRI)
    • Computerized tomography (CT) scan 2 These investigations are necessary to rule out underlying malignancies, as AN can be a paraneoplastic syndrome associated with various types of cancer, including gastric, lung, ovarian, and breast carcinomas 3, 4, 5.

Importance of Screening

Screening for metabolic syndrome and malignancies is crucial in patients with AN, as it can be a red flag for underlying pathologies 3. The combination of AN and skin tags is more often associated with metabolic syndrome, but AN may also be a marker of internal malignancy 3, 5.

Diagnostic Approach

A comprehensive diagnostic approach is necessary to determine the underlying cause of AN, including clinical evaluation, laboratory tests, and imaging studies 2, 6. This approach will help to exclude malignancy and identify any associated conditions, such as metabolic syndrome or other systemic diseases.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

An approach to acanthosis nigricans.

Indian dermatology online journal, 2014

Research

Acanthosis Nigricans - A Two-Sided Coin: Consider Metabolic Syndrome and Malignancies!

Open access Macedonian journal of medical sciences, 2019

Research

Malignant acanthosis nigricans: a review.

The Journal of dermatologic surgery and oncology, 1980

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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