What are the most common causative pathogens in acute cholecystitis?

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From the Guidelines

The most common causative pathogens in acute cholecystitis are gram-negative aerobes, such as Escherichia coli and Klebsiella pneumoniae, and anaerobes, especially Bacteroides fragilis. These pathogens are the primary cause of infection in acute cholecystitis, and their identification is crucial for guiding empiric antibiotic therapy 1.

Key Pathogens

  • Escherichia coli
  • Klebsiella pneumoniae
  • Bacteroides fragilis

Empiric Antibiotic Therapy

The choice of empiric antibiotic regimen should be based on the most frequently isolated germs, taking into consideration local trends of antibiotic resistance 1. In patients with complicated acute cholecystitis, initial empiric antibiotic therapy is necessary, and the decision for empiric antimicrobial management depends mainly on the presumed pathogens involved and risk factors for major resistance patterns and disease severity 1.

Clinical Considerations

Elderly patients, especially those from institutions such as nursing homes or geriatric hospitals, can be colonized by multidrug-resistant organisms, posing a particular challenge 1. In these patients, intraoperative cultures should be performed to reassess the antibiotic regimen, and complex regimens with broader spectra may be recommended 1. Early correct empirical antimicrobial therapy has a significant impact on the outcome in patients with sepsis, and recent international guidelines recommend intravenous antibiotics within the first hour after severe sepsis and septic shock are recognized 1.

From the Research

Causative Pathogens in Acute Cholecystitis

The most common causative pathogens in acute cholecystitis include:

  • Escherichia coli 2, 3, 4, 5
  • Klebsiella spp. 2, 3, 5
  • Streptococcus spp. 2, 5, 6
  • Enterococcus spp. 2, 3, 4, 5
  • Clostridium spp. 2
  • Enterobacter 3
  • Bacteroides fragilis and clostridia (anaerobes) 5
  • Streptococcus gallolyticus subsp. pasteurianus (rare) 6

Bile Bacterial Colonization

Bile bacterial colonization rates in patients with acute cholecystitis range from 33.6% to 60% 2, 4, 5. The presence of bactibilia is particularly prevalent in the elderly and in cases of recurrent cholecystitis 5.

Antibiotic Susceptibilities

Antibiotic susceptibilities vary among the different microorganisms, with:

  • Second-generation cephalosporin (cefotetan) being effective against Gram-negative microorganisms 3
  • Vancomycin and teicoplanin being effective against Enterococcus 3
  • A combination of broad-spectrum beta-lactam antibiotics and nitroimidazole providing appropriate coverage 5

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Updates on Antibiotic Regimens in Acute Cholecystitis.

Medicina (Kaunas, Lithuania), 2024

Research

Suggested use of empirical antibiotics in acute cholecystitis based on bile microbiology and antibiotic susceptibility.

HPB : the official journal of the International Hepato Pancreato Biliary Association, 2023

Research

Cholecystitis--etiology and treatment--microbiological aspects.

Scandinavian journal of gastroenterology. Supplement, 1984

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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