What are the diagnostic criteria for adrenal insufficiency (Addison's disease)?

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Last updated: March 5, 2025View editorial policy

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From the Guidelines

Diagnosing adrenal insufficiency requires a combination of clinical assessment and laboratory testing, starting with the measurement of morning serum cortisol and ACTH levels, as recommended by the American Society of Clinical Oncology 1. The initial evaluation should include measurement of morning serum cortisol and ACTH levels.

  • Morning serum cortisol level
  • ACTH level Followed by an ACTH stimulation test, which is the gold standard diagnostic test, as suggested by the Journal of Internal Medicine 1. For the ACTH stimulation test, synthetic ACTH (cosyntropin/Synacthen) is administered at a dose of 250 μg intravenously, with cortisol levels measured at baseline, 30 minutes, and 60 minutes after administration.
  • A normal response is a peak cortisol level above 18-20 μg/dL (500-550 nmol/L); failure to reach this threshold suggests adrenal insufficiency. Primary adrenal insufficiency is characterized by low cortisol with elevated ACTH levels, while secondary insufficiency shows low cortisol with low or inappropriately normal ACTH levels. Additional testing may include electrolytes (looking for hyponatremia and hyperkalemia), blood glucose (hypoglycemia), and adrenal antibodies to determine etiology. In suspected secondary adrenal insufficiency, pituitary imaging with MRI is recommended. Patients with symptoms of adrenal crisis (severe hypotension, shock, altered mental status) should receive immediate treatment with hydrocortisone 100 mg IV before completing diagnostic testing, as delaying treatment can be life-threatening, as emphasized by the Journal of Internal Medicine 1. The insulin tolerance test and metyrapone test are alternative diagnostic options but are less commonly used due to safety concerns and availability issues. It is essential to note that treatment of suspected acute adrenal insufficiency should never be delayed by diagnostic procedures, as stated in the Journal of Internal Medicine 1. The diagnostic test for primary PAI should be paired measurement of serum cortisol and plasma ACTH, and in equivocal cases, a synacthen (tetracosactide) stimulated peak serum cortisol <500 nmol L1 is diagnostic of PAI, as recommended by the Journal of Internal Medicine 1.

From the Research

Diagnostic Criteria for Adrenal Insufficiency

The diagnostic criteria for adrenal insufficiency, also known as Addison's disease, involve a combination of clinical suspicion, laboratory tests, and imaging studies. The diagnosis is often challenging due to the non-specific symptoms and variable clinical course [ 2, 3, 4, 5, 6 ].

Clinical Presentation

The clinical presentation of adrenal insufficiency can range from mild, non-specific symptoms such as fatigue, to life-threatening adrenal crisis with hemodynamic instability [ 2 ]. The symptoms can be insidious, making it essential to have a high index of suspicion [ 5 ].

Laboratory Tests

The diagnosis of adrenal insufficiency is made by demonstrating low basal and/or stimulated serum cortisol [ 4, 6 ]. The cosyntropin stimulation test is a specific test used to confirm the diagnosis [ 2, 6 ]. The insulin tolerance test is the gold standard for secondary adrenal insufficiency [ 6 ].

Diagnostic Clues

Important diagnostic clues include:

  • Hyponatremia
  • Hyperkalemia
  • Hypotension refractory to fluids and vasopressors [ 5 ]
  • Low baseline cortisol (<100 nmol/L) alongside raised adrenocorticotropic hormone (ACTH) [ 6 ]

Investigation and Management

A strategic approach to investigation can help identify the underlying cause of adrenal insufficiency [ 6 ]. Patient education and empowerment are crucial in managing the condition [ 4, 6 ]. Regular monitoring for features of under- and over-replacement is essential during follow-up [ 4 ].

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

[Diagnosis and therapy of patients with adrenocortical insufficiency].

Nederlands tijdschrift voor geneeskunde, 1998

Research

Adrenal insufficiency - recognition and management.

Clinical medicine (London, England), 2017

Research

Diagnosis and management of adrenal insufficiency.

Clinical medicine (London, England), 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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