What are the most current guidelines for the workup of sepsis (Systemic Inflammatory Response Syndrome)?

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Last updated: March 9, 2025 • View editorial policy

From the Guidelines

The most current guidelines for the workup of sepsis recommend following the Surviving Sepsis Campaign's Hour-1 Bundle, which includes administering broad-spectrum antibiotics within one hour of sepsis recognition, measuring lactate levels, obtaining blood cultures before antibiotics, and beginning rapid fluid resuscitation with 30 mL/kg crystalloid for hypotension or lactate ≥4 mmol/L 1.

Key Components of Sepsis Workup

  • Initial assessment should include measuring lactate levels, obtaining blood cultures before antibiotics, and administering broad-spectrum antibiotics within one hour of sepsis recognition 1
  • For diagnosis, use the Sequential Organ Failure Assessment (SOFA) score or quick SOFA (qSOFA) criteria to identify patients at risk 2
  • Laboratory workup should include complete blood count, comprehensive metabolic panel, coagulation studies, and cultures from all potential infection sources
  • Imaging should be directed by suspected infection source ### Antibiotic Therapy
  • Empiric antibiotic therapy should cover likely pathogens based on infection site, with vancomycin plus piperacillin-tazobactam or meropenem commonly used for broad coverage 1
  • Antibiotics should be administered within one hour of sepsis recognition and de-escalated once pathogens are identified 1
  • The antimicrobial regimen should be reassessed daily for potential de-escalation 1 ### Hemodynamic Support
  • Norepinephrine is the first-line vasopressor for fluid-refractory shock, with a target mean arterial pressure of 65 mmHg 2
  • Fluid therapy is needed to improve microvascular blood flow through an increased cardiac output as an essential part of the treatment of sepsis
  • A fluid challenge should incorporate four determinant elements: crystalloid solutions, rapid infusion, goal of increasing systemic arterial pressure, and avoidance of pulmonary edema 2 ### Ongoing Management
  • Ongoing management includes source control, daily reassessment of antibiotic therapy, and monitoring for organ dysfunction
  • The duration of therapy typically ranges from 7 to 10 days, but may be longer in patients with a slow clinical response, undrainable foci of infection, or immunologic deficiencies 1

From the Research

Current Guidelines for Sepsis Workup

The current guidelines for the workup of sepsis, also known as Systemic Inflammatory Response Syndrome, involve several key steps:

  • Early goal-directed resuscitation of the septic patient during the first 6 hours after recognition 3
  • Appropriate diagnostic studies to ascertain causative organisms before starting antibiotics 3
  • Early administration of broad-spectrum antibiotic therapy 3, 4
  • Reassessment of antibiotic therapy with microbiology and clinical data to narrow coverage, when appropriate 3
  • A usual 7-10 days of antibiotic therapy guided by clinical response 3

Fluid Resuscitation and Vasopressor Therapy

Fluid resuscitation and vasopressor therapy are crucial in managing sepsis:

  • Liberal and restrictive fluid strategies are comparable in efficacy, but restrictive fluid regimens have a lower incidence of fluid overload 5
  • Balanced crystalloids are safer and more effective than normal saline 5
  • Albumin replacement is safe and effective in reducing mortality in patients with sepsis and septic shock 5
  • Vasopressor therapy is indicated when fluid resuscitation fails to restore adequate mean arterial pressure and organ perfusion 4

Antibiotic Therapy and Timing

The timing of antibiotic therapy is critical in sepsis management:

  • Early antibiotic therapy can improve clinical outcomes and should be given within one hour of suspected sepsis 4
  • There is limited evidence on the optimal timing of broad-spectrum antibiotics in adult patients with severe sepsis in the ED pre-ICU admission 6
  • High-dose meropenem may be effective in critically ill patients with sepsis and septic shock, but further research is needed to confirm its safety and efficacy 7

Other Considerations

Other important considerations in sepsis management include:

  • Source control with attention to the method that balances risks and benefits 3
  • Stress-dose steroid therapy for septic shock 3
  • Use of recombinant activated protein C in patients with severe sepsis and high risk for death 3
  • Maintenance of blood glucose <150 mg/dL after initial stabilization 3

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.