What is the negative predictive value of a Clostridioides difficile (C. diff) test?

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Last updated: February 9, 2025View editorial policy

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From the Guidelines

The negative predictive value of a Clostridioides difficile (C. diff) test is typically >99%.

Details of the Evidence

  • The high negative predictive value is based on the use of highly sensitive testing strategies, such as 2-stage algorithms or stand-alone NAATs 1.
  • This means that a single negative test result has a very high likelihood of accurately ruling out CDI.
  • The evidence suggests that repeat testing within a 7-day period has a low diagnostic yield of approximately 2% 1.
  • However, repeat testing may be considered in certain situations, such as epidemic settings or for symptomatic patients with a high clinical suspicion of CDI but a negative initial test result 1.
  • It is also important to note that recurrent CDI occurs commonly, and repeat testing should be considered in patients who experience a recurrence of symptoms following successful treatment 1.

From the Research

Negative Predictive Value of C. diff Tests

The negative predictive value (NPV) of a C. diff test is a measure of the probability that a patient with a negative test result truly does not have the infection.

  • The NPV of the C. DIFF QUIK CHEK COMPLETE test was found to be 91.7% 2 and 89.1% for the Quik Chek Complete CDT 3.
  • The NPV of the Tox A/B EIA was found to be 85.8% 2.
  • The NPV of the BD MAX Cdiff assay was found to be 100% for all patients, pediatric patients, and adult patients 4.
  • The NPV of the Xpert CD assay was found to be 98.7% 3.
  • The NPV of the glutamate dehydrogenase (GDH) test was found to be 99.4% 3 and 99.3% 5.

Comparison of NPV Values

The NPV values of different C. diff tests vary, with the BD MAX Cdiff assay having the highest NPV of 100%, indicating that a negative result can reliably rule out the infection.

  • The C. DIFF QUIK CHEK COMPLETE test and the Xpert CD assay also have high NPV values, indicating good performance in ruling out the infection.
  • The Tox A/B EIA has a lower NPV, indicating that a negative result may not always rule out the infection.
  • The GDH test has a high NPV, indicating that it can be a useful screening tool for C. diff infection.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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