What are the laboratory findings in Graves' disease (Hyperthyroidism)?

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Last updated: March 11, 2025 • View editorial policy

From the Guidelines

Graves' disease is characterized by laboratory findings of suppressed thyroid-stimulating hormone (TSH) levels, typically less than 0.1 mIU/L, and elevated free thyroxine (FT4) and free triiodothyronine (FT3) levels, with positive thyroid-stimulating immunoglobulin (TSI) or thyroid-stimulating hormone receptor antibodies (TRAb) confirming the autoimmune nature of the condition. The primary tests for diagnosing Graves' disease include:

  • Thyroid-stimulating hormone (TSH) levels, which are usually suppressed (<0.1 mIU/L) 1
  • Elevated free thyroxine (FT4) and free triiodothyronine (FT3) levels
  • Thyroid-stimulating immunoglobulin (TSI) or thyroid-stimulating hormone receptor antibodies (TRAb), which are specific markers for Graves' disease Additional tests may include:
  • Thyroid peroxidase antibodies (TPOAb) and thyroglobulin antibodies (TgAb), which are often elevated in autoimmune thyroid conditions
  • Complete blood count and liver function tests, which may be ordered before initiating treatment
  • Radioactive iodine uptake scan, which can help confirm diagnosis by showing diffuse increased uptake throughout the thyroid gland, although it is not a laboratory test 1. These tests collectively help differentiate Graves' disease from other causes of hyperthyroidism, as the presence of TSI/TRAb antibodies is specific to Graves' disease and indicates autoimmune stimulation of the thyroid gland causing excessive hormone production. It is essential to note that the diagnosis and management of Graves' disease should be based on the most recent and highest-quality evidence, and treatment should be individualized to minimize morbidity, mortality, and improve quality of life 1.

From the Research

Laboratory Findings in Graves' Disease

The laboratory findings in Graves' disease, also known as hyperthyroidism, include:

  • Low serum TSH concentrations, which is the most reliable indicator of thyroid function 2
  • Elevated serum levels of FT4, which is a more accurate measure of thyroid function than total T4 (TT4) 2
  • Elevated serum levels of FT3, although its measurement is not as significant as T3 2
  • Presence of serum autoantibodies against thyroid tissues, such as TgAb and TPOAb 2
  • Presence of TSH receptor antibodies, which differentiate Graves' disease from Hashimoto's thyroiditis 2, 3, 4
  • Elevated serum levels of TRAb, which can be measured by radioreceptor assay or bioassay using cultured porcine thyroid cells 2, 3, 4

Diagnostic Tests

The diagnostic tests for Graves' disease include:

  • Measurement of serum TSH, FT4, and FT3 levels 2, 5, 6
  • Detection of serum autoantibodies against thyroid tissues, such as TgAb and TPOAb 2
  • Measurement of TSH receptor antibodies, such as TRAb and TSAb 2, 3, 4
  • Thyroid ultrasonography, which can help diagnose Graves' disease and monitor its progression 3

References

Research

[Thyroid function tests].

Rinsho byori. The Japanese journal of clinical pathology, 2001

Research

Diagnosis and management of Graves disease: a global overview.

Nature reviews. Endocrinology, 2013

Research

Diagnosis and management of Graves' disease.

CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne, 2003

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.