What are the guidelines for anal pap smears in Human Immunodeficiency Virus (HIV) negative men?

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Last updated: September 3, 2025View editorial policy

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Anal Pap Screening Guidelines for HIV-Negative Men

Current guidelines do not recommend routine anal Pap smear screening for HIV-negative men due to insufficient evidence supporting its effectiveness in reducing anal cancer morbidity and mortality. 1

Risk Assessment for Anal Cancer in HIV-Negative Men

The risk of anal cancer varies significantly based on specific risk factors:

  • General population: 1-2 cases per 100,000 person-years 1
  • HIV-negative MSM: 14 cases per 100,000 person-years 1
  • HIV-positive MSM: 80-131 cases per 100,000 person-years 1, 2

Key Risk Factors in HIV-Negative Men

  • History of receptive anal intercourse 1, 2
  • Previous HPV-related genital dysplasia or cancer 2
  • Presence of genital warts 2
  • History of immunosuppression (non-HIV related) 3

Current Screening Recommendations

Digital Anorectal Examination (DARE)

  • For HIV-negative MSM with history of receptive anal intercourse: Annual DARE may be useful to detect masses on palpation 1
  • DARE is acceptable to patients and has a low risk for adverse outcomes 1
  • No specific guidelines exist for initiation age or examination intervals 1

Anal Cytology (Anal Pap)

  • Not routinely recommended for HIV-negative men in the general population 1, 4
  • Data are insufficient to support routine anal cancer screening with anal cytology among HIV-negative men 1
  • Some clinical centers perform anal cytology for high-risk populations (primarily HIV-positive individuals and MSM with history of receptive anal intercourse) 1

Considerations for Selective Screening

If considering anal Pap testing for high-risk HIV-negative MSM, important limitations to consider:

  • Sensitivity of anal cytology in HIV-negative males ranges from 26-47% 3
  • Specificity ranges from 81-92% 3
  • Health centers should only initiate cytology-based screening if referrals to high-resolution anoscopy (HRA) and biopsy are available 1

Prevention Strategies

HPV Vaccination

  • Recommended for all males aged 9-21 years 1
  • Males aged 22-26 years should also be vaccinated if not previously vaccinated 1
  • HPV vaccination is highly effective in preventing infection with HPV subtypes responsible for approximately 70% of anal cancers 1
  • No therapeutic effect on pre-existing anal dysplasia 1, 2

Management of Abnormal Findings

If anal cytology is performed and abnormal results are found:

  1. Referral for high-resolution anoscopy (HRA) is recommended 1
  2. Biopsy of any abnormal areas should be performed 1
  3. Treatment should be based on biopsy results 1, 2

Research Gaps

  • More evidence is needed regarding the natural history of anal intraepithelial neoplasia in HIV-negative men 1
  • Optimal screening methods and target populations remain undefined 1, 4
  • Ongoing clinical trials are investigating whether treatment of high-grade squamous intraepithelial lesions (HSIL) reduces anal cancer incidence 1

Conclusion

While anal cancer screening with anal Pap tests is being studied in high-risk populations, current guidelines do not support routine screening for HIV-negative men. Annual digital anorectal examination may be considered for HIV-negative MSM with a history of receptive anal intercourse, but more research is needed before broader screening recommendations can be made.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Anal Cancer Screening and Prevention

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Anal dysplasia screening: an evidence-based analysis.

Ontario health technology assessment series, 2007

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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