What is the purpose of a Pap (Papanicolaou) smear procedure?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: March 12, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

The primary purpose of a Pap smear procedure is to screen for cervical cancer by collecting cells from the cervix to check for abnormalities, with the goal of detecting precancerous changes before they develop into cervical cancer, allowing for early intervention and potentially life-saving treatment. The procedure involves collecting cells from the cervix using a small brush or spatula, which are then sent to a laboratory for analysis 1. According to the American Cancer Society, women should begin regular Pap tests at age 21 and continue every three years until age 65 if results remain normal, with the option of testing every five years when combined with HPV testing for women aged 30-65 1. Some key points to consider when undergoing a Pap smear include:

  • Avoiding sexual intercourse, douching, or using vaginal medications for 24-48 hours before the test
  • Rescheduling the appointment if menstruating
  • Understanding that a positive high-risk HPV test or an abnormal cervical cytology test is not indicative of cervical cancer, but rather requires appropriate follow-up to ensure that cervical abnormalities do not progress 1
  • Recognizing that a normal Pap test and a positive high-risk HPV test indicate an HPV infection of the cervix, which increases the likelihood of developing cell changes that could lead to cervical cancer over time, and therefore requires follow-up evaluation 1. Overall, the Pap smear procedure is a crucial screening test for cervical cancer that can detect precancerous changes and allow for early intervention, making it a potentially life-saving test 1.

From the Research

Purpose of a Pap Smear Procedure

The purpose of a Pap smear procedure is to screen for cervical cancer and detect precancerous lesions.

  • The test involves collecting cells from the cervix and examining them for abnormal cell changes 2.
  • The goal is to identify potentially cancerous cells before they become malignant, allowing for early intervention and treatment 3.
  • A Pap smear can also detect human papillomavirus (HPV) changes, which can increase the risk of developing cervical cancer 4, 5.

Detection of Abnormal Cell Changes

The Pap smear procedure is designed to detect abnormal cell changes, including:

  • Precancerous lesions, such as dysplasia 3
  • HPV changes, which can increase the risk of developing cervical cancer 4, 5
  • Atypia, which can indicate abnormal cell growth 3

Importance of Accurate Sampling

Accurate sampling of the cervix is crucial for a reliable Pap smear result.

  • The sampling device should be designed to collect cells from the complete transformation zone (TZ) and the squamocolumnar junction (SCJ) 2.
  • Immediate wet fixation of the smear is also important to preserve the cells and ensure accurate results 2.

Follow-up and Screening Intervals

The follow-up and screening intervals for Pap smears depend on various factors, including:

  • The results of the Pap smear and HPV testing 4, 5
  • The patient's age and medical history 4
  • The presence of abnormal cell changes or HPV changes 3, 5
  • Guidelines recommend screening for cervical cancer among women 30 years or older 3 years after a normal Pap test result or a combined normal screening result (normal Pap/negative HPV test results) 4.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.