What type of fluid is not lined by mesothelial (mesothelial) cells: peritoneal fluid, pericardial fluid, cerebrospinal fluid (CSF), or pleural fluid?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: September 6, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Cerebrospinal Fluid (CSF) is Not Lined by Mesothelial Cells

The correct answer is C. CSF (cerebrospinal fluid) is the only fluid among the options that is not lined by mesothelial cells.

Mesothelial Cell Distribution in Body Cavities

Mesothelial cells form a protective monolayer that lines the serous cavities of the body, including:

  • Pleural cavity - containing pleural fluid
  • Pericardial cavity - containing pericardial fluid
  • Peritoneal cavity - containing peritoneal fluid

These specialized epithelial cells create a non-adhesive barrier that protects against physical and biochemical damage in these cavities 1.

Cerebrospinal Fluid: Different Cellular Origin

Unlike the other fluids mentioned, cerebrospinal fluid is:

  • Produced primarily by the choroid plexus epithelium and ependymal cells of the ventricles 2
  • Contained within the subarachnoid space of the central nervous system
  • Not lined by mesothelial cells but rather by specialized neural tissue

The CSF flows through the ventricular system and subarachnoid space, which has a completely different embryological origin and cellular composition than the mesothelium-lined cavities 3.

Characteristics of Mesothelial-Lined Cavities

Mesothelial cells lining the pleural, pericardial, and peritoneal cavities share important features:

  • Express both epithelial markers (cytokeratins) and mesenchymal markers (vimentin) 1
  • Produce glycocalyx, a hydrophilic protective barrier consisting mainly of glycosaminoglycans 1
  • Play key roles in fluid transport and inflammation 4
  • Secrete various cytokines including G-CSF, GM-CSF, M-CSF, IL-1, and IL-6 5
  • Regulate electrolyte and fluid transport across serosal membranes 4

Diagnostic Implications

The distinction between mesothelial and non-mesothelial linings has important diagnostic implications:

  • Cytological examination of pleural, pericardial, and peritoneal fluids may reveal mesothelial cells that can be identified by specific immunohistochemical markers 3
  • Malignant mesothelioma specifically arises from mesothelial cells of the pleura, peritoneum, or pericardium, but not from the CNS 3
  • Immunohistochemistry markers like calretinin, keratins 5/6, and WT1 are used to identify mesothelial cells in diagnostic samples 3

Clinical Relevance

Understanding the different cellular linings of body cavities is essential for:

  • Interpreting cytological findings in fluid samples
  • Differentiating between primary malignancies (like mesothelioma) and metastatic disease
  • Understanding the pathophysiology of effusions in different body cavities
  • Developing targeted treatments for conditions affecting specific body cavities

The mesothelium should be considered a specialized organ with distinct functions rather than just a membrane 1, while the cerebrospinal fluid system has its own unique cellular environment and functions related to the central nervous system.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.