Is a booster dose of the JYNNEOS (smallpox and monkeypox vaccine) recommended for monkeypox prevention?

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Last updated: September 10, 2025View editorial policy

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JYNNEOS Booster for Monkeypox Prevention

A booster dose of JYNNEOS vaccine is not currently recommended for monkeypox prevention after completion of the standard 2-dose series. According to the most recent evidence, the 2-dose series provides effective protection without the need for additional doses 1.

Current Vaccination Recommendations

The JYNNEOS vaccine is administered as a 2-dose series with doses given 28 days apart. This vaccination approach is supported by multiple lines of evidence:

  • The vaccine is licensed as a 2-dose series (0.5 mL per dose subcutaneously or 0.1 mL per dose intradermally) 2, 3
  • Completion of both doses is necessary for optimal protection against monkeypox virus infection 4
  • Recent data from 2024 indicates that after receiving the complete 2-dose series, additional doses are not currently recommended 1

Vaccine Effectiveness

The effectiveness of the 2-dose JYNNEOS vaccine series has been well-documented:

  • A case-control study conducted across 12 U.S. jurisdictions showed that the adjusted vaccine effectiveness was:

    • 75.2% for partial vaccination (1 dose)
    • 85.9% for full vaccination (2 doses) 3
  • Full vaccination effectiveness was similar across different administration routes:

    • 88.9% for subcutaneous administration
    • 80.3% for intradermal administration
    • 86.9% for heterologous (mixed) administration 3

Duration of Protection

Current evidence suggests that immunity from the 2-dose series is not waning significantly:

  • Among breakthrough infections in fully vaccinated individuals, the median interval between the second dose and illness onset was:

    • 363 days for those who received 2 intradermal doses
    • 263 days for those who received 2 subcutaneous doses 1
  • These disparate time intervals suggest that immunity is not waning in a way that would necessitate a booster dose at this time 1

Breakthrough Infections

While the vaccine is highly effective, breakthrough infections can occur:

  • During May 2022-May 2024,271 mpox cases among fully vaccinated persons were reported to CDC from 27 U.S. jurisdictions 1
  • These infections are estimated to have occurred in less than 1% of fully vaccinated persons 1
  • Importantly, infections among fully vaccinated persons resulted in less severe disease compared to infections in unvaccinated individuals 1

Special Considerations

For laboratory workers or healthcare personnel with potential occupational exposure to orthopoxviruses:

  • Historical guidance for those working with vaccinia virus, recombinant vaccinia viruses, or other nonvariola Orthopoxviruses recommended revaccination every 10 years 5
  • For those at risk for exposure to more virulent nonvariola Orthopoxviruses (e.g., monkeypox), empiric revaccination every 3 years was considered 5

However, these recommendations predated the current JYNNEOS vaccine and were primarily focused on older vaccinia-based vaccines in laboratory settings.

Safety Profile

The JYNNEOS vaccine has demonstrated a favorable safety profile:

  • The most common adverse events are nonserious and include injection site reactions 6
  • Serious adverse events are rare in adults 6
  • Adverse events are reported at similar rates for both intradermal and subcutaneous administration 6

Conclusion

Based on the most recent evidence, persons recommended to receive the JYNNEOS vaccine should complete the standard 2-dose series, regardless of the route of administration. At this time, additional booster doses are not recommended for monkeypox prevention 1.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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