Is it necessary to administer Vitamin K2 with Vitamin D3 (Cholecalciferol)?

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Last updated: March 14, 2025 • View editorial policy

From the Guidelines

Vitamin K2 supplementation is not necessary when taking Vitamin D3, but it may be beneficial for directing calcium to bones and reducing arterial calcification risk. When considering the administration of Vitamin K2 with Vitamin D3, it's essential to understand the role of each vitamin. Vitamin D3, or cholecalciferol, is crucial for calcium absorption, and its supplementation is recommended to maintain serum 25(OH) D levels at 30 ng/ml or higher for bone health 1. The study by the NCCN task force report on bone health in cancer care suggests that vitamin D3 could be more effective in raising 25(OH) D concentrations and maintaining those levels for a longer time when longer dosing intervals are employed 1. However, the same study does not mention the necessity of administering Vitamin K2 with Vitamin D3. Some key points to consider when supplementing with Vitamin D3 include:

  • The typical recommended dosage of vitamin D3 is 800 to 1000 IU daily, with a caveat that many patients may need more than the recommended amount and should be repleted based on serum 25(OH) D level 1.
  • Vitamin D toxicity is uncommon but may occur with daily doses of more than 50,000 IU per day that produce 25(OH) D levels larger than 150 ng/mL 1. While the evidence does not necessitate the administration of Vitamin K2 with Vitamin D3, taking them together may have synergistic effects on bone health and potentially reduce the risk of arterial calcification. It is crucial to consult a healthcare provider before taking any supplements, especially for individuals on blood-thinning medications like warfarin, as vitamin K2 may interfere with these medications. In terms of dosage, a typical range for vitamin K2 is 100-200 mcg (preferably as MK-7 form) with 1000-5000 IU of vitamin D3 daily, though individual needs may vary.

From the Research

Vitamin K2 and Vitamin D3 Administration

  • The administration of Vitamin K2 with Vitamin D3 (Cholecalciferol) has been studied in various research papers 2, 3, 4.
  • A study published in 2000 found that the combined administration of vitamin D3 and vitamin K2 increased bone mineral density (BMD) of the lumbar spine in postmenopausal women with osteoporosis compared to calcium administration alone 2.
  • However, a 2021 study found that vitamin K2 supplementation as an add-on to calcium and vitamin D supplementation did not affect biochemical markers of bone turnover, bone mineral density, or bone microarchitecture in postmenopausal women with osteopenia 3.
  • Another study published in 2024 highlighted the importance of vitamin K and the combination of vitamins K and D for calcium metabolism and bone health, suggesting that an adequate supply of vitamin K, on top of an optimal vitamin D status, seems to add to the benefit of maintaining bone health 4.

Vitamin D3 Supplementation

  • Research has also focused on vitamin D3 supplementation, with a 2014 study recommending that vitamin D3 be used for supplementation over vitamin D2, and concluding that single vitamin D3 doses ≥300,000 IU are most effective at improving vitamin D status and suppressing parathyroid hormone (PTH) concentrations for up to 3 months 5.
  • A 2024 review argued for a daily dose of 2000 international units (50 µg) of vitamin D for adults in the general population, citing evidence that such a dose may improve some health outcomes and is sufficient to raise and maintain serum 25(OH)D concentrations above 50 nmol/L (20 ng/mL) and above 75 nmol/L (30 ng/mL) in >99% and >90% of the general adult population, respectively 6.

Interaction between Vitamins K and D

  • The interaction between vitamins K and D has been explored, with evidence suggesting that vitamin K-dependent proteins, such as osteocalcin and matrix Gla-protein (MGP), play a crucial role in bone health 4.
  • The combined action of vitamins K and D may contribute to the maintenance of bone health by promoting bone mineralization and reducing the risk of osteoporosis 4.

References

Guideline

nccn task force report: bone health in cancer care.

Journal of the National Comprehensive Cancer Network : JNCCN, 2009

Research

Effect of combined administration of vitamin D3 and vitamin K2 on bone mineral density of the lumbar spine in postmenopausal women with osteoporosis.

Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 2000

Research

The effect of vitamin MK-7 on bone mineral density and microarchitecture in postmenopausal women with osteopenia, a 3-year randomized, placebo-controlled clinical trial.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2021

Research

Large, single-dose, oral vitamin D supplementation in adult populations: a systematic review.

Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists, 2014

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.