What is a Neprilysin (NEP) inhibitor?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: March 16, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

Neprilysin (NEP) inhibitors are a class of medications that block the enzyme neprilysin, which breaks down natriuretic peptides, bradykinin, adrenomedullin, and other vasoactive peptides, and the most clinically important NEP inhibitor is sacubitril, combined with valsartan in the medication Entresto, primarily used to treat heart failure with reduced ejection fraction (HFrEF) as shown in the 2022 AHA/ACC/HFSA guideline 1. The mechanism of action of NEP inhibitors involves increasing levels of natriuretic peptides, which promote vasodilation, natriuresis, and diuresis, while reducing sympathetic tone and inhibiting cardiac remodeling. Key points to consider when using NEP inhibitors include:

  • The typical starting dose of sacubitril-valsartan is 24/26 mg twice daily, which can be titrated up to 97/103 mg twice daily as tolerated 1.
  • Patients should be monitored for hypotension, hyperkalemia, and renal dysfunction.
  • NEP inhibitors should not be used concurrently with ACE inhibitors due to increased risk of angioedema, and a 36-hour washout period is required when switching from an ACE inhibitor. The use of NEP inhibitors, such as sacubitril-valsartan, has been shown to significantly reduce hospitalizations and mortality in heart failure patients compared to ACE inhibitors alone, as demonstrated in the PARADIGM-HF trial 1. Overall, NEP inhibitors, particularly sacubitril-valsartan, are a valuable treatment option for patients with HFrEF, offering improved outcomes and simplified management, as supported by the 2022 AHA/ACC/HFSA guideline 1.

From the FDA Drug Label

Sacubitril and valsartan contains a neprilysin inhibitor, sacubitril, and an angiotensin receptor blocker, valsartan. Sacubitril and valsartan inhibits neprilysin (neutral endopeptidase; NEP) via LBQ657, the active metabolite of the prodrug sacubitril, and blocks the angiotensin II type-1 (AT1) receptor via valsartan

A Neprilysin (NEP) inhibitor is a type of drug that inhibits the action of neprilysin, also known as neutral endopeptidase.

  • Key points:
    • Neprilysin is an enzyme involved in the breakdown of certain peptides, such as natriuretic peptides.
    • Inhibition of neprilysin increases the levels of these peptides, which can have beneficial effects on the cardiovascular system.
    • Sacubitril is an example of a neprilysin inhibitor, which is used in combination with valsartan to treat heart failure 2.

From the Research

Definition of Neprilysin (NEP) Inhibitor

  • A Neprilysin (NEP) inhibitor is a type of drug that inhibits the action of neprilysin, an endopeptidase that metabolizes different vasoactive peptides, including natriuretic peptides, bradykinin, and angiotensin II 3.
  • NEP inhibitors, such as sacubitril, work by increasing the levels of these peptides, which can help to promote diuresis, natriuresis, and vasodilatation 3.

Mechanism of Action

  • The inhibition of NEP increases the levels of natriuretic peptides, which can help to counteract the effects of the renin-angiotensin-aldosterone system (RAAS) 4.
  • The increased levels of natriuretic peptides can also help to improve cardiac remodeling and reduce mortality in patients with heart failure with reduced ejection fraction (HFrEF) 4.

Examples of NEP Inhibitors

  • Sacubitril/valsartan is a first-in-class angiotensin receptor-neprilysin inhibitor (ARNI) that has been shown to be effective in reducing morbidity and mortality in patients with HFrEF 5, 6.
  • Sacubitril/valsartan has been recommended in clinical practice guidelines as a replacement for angiotensin-converting-enzyme inhibitors (ACE-I) in patients with HFrEF who remain symptomatic despite optimal medical therapy 7.

Clinical Benefits

  • NEP inhibitors, such as sacubitril/valsartan, have been shown to reduce mortality and hospitalization rates in patients with HFrEF 3, 4, 7.
  • They have also been shown to improve cardiac remodeling and quality of life in these patients 4.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.