What are the signs of a hemolytic reaction?

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From the Guidelines

A hemolytic reaction is characterized by signs such as fever, chills, back or flank pain, and a feeling of unease or anxiety, and can be accompanied by hyperhemolysis, which is a severe hemolysis causing the hemoglobin to drop below pretransfusion levels, as noted in the 2020 guidelines for sickle cell disease: transfusion support 1. The most common signs of a hemolytic reaction include:

  • Fever
  • Chills
  • Back or flank pain
  • A feeling of unease or anxiety
  • Shortness of breath
  • Chest pain
  • Rapid heart rate Visible changes can include:
  • Jaundice (yellowing of the skin and eyes)
  • Dark or red-colored urine (hemoglobinuria)
  • Hypotension or shock in severe cases These reactions often occur during or shortly after blood transfusions but can also result from certain medications, autoimmune conditions, or infections. Laboratory findings typically show:
  • Decreased hemoglobin levels
  • Elevated bilirubin
  • Increased lactate dehydrogenase (LDH)
  • Reduced haptoglobin
  • The presence of schistocytes (fragmented red blood cells) on a peripheral blood smear It is essential to monitor patients for adverse events or reactions during transfusions, including heart rate, blood pressure, temperature, and respiratory rate, as per local guidelines, and to stop the transfusion and contact the laboratory immediately if any signs of a transfusion reaction occur, such as tachycardia, rash, breathlessness, hypotension, or fever 1. In patients with sickle cell disease, hyperhemolysis can occur with no identifiable antibody and a negative direct antiglobulin test, and recognition is critical, as additional transfusions should be avoided if possible, to prevent worsening of the hemolysis and potential induction of multiorgan failure and death 1.

From the Research

Signs of a Hemolytic Reaction

  • The signs and symptoms of acute hemolytic transfusion reactions include anaemia, fever, haemoglobinuria, and jaundice 2
  • Subjective responses of pain, restlessness, nausea, skin flushing, dyspnoea, and shock are mediated by cleavage products of complement (C3a, C5a) activated by red cell antigen-antibody reaction 2
  • Severe hemolysis, acute renal failure, and disseminated intravascular coagulation can occur in acute hemolytic transfusion reactions 3
  • Hemoglobinemia and hemoglobinuria can result in disseminated intravascular coagulation (DIC), acute renal failure, shock, and sometimes death 4
  • Delayed hemolytic transfusion reactions can cause an additional bystander hemolysis of the patient's RBCs 5

Clinical Presentation

  • Acute hemolytic transfusion reactions can be defined as the occurrence after transfusion of measurably increased destruction of red cells, of donor or recipient, by alloantibodies 2
  • The clinical consequences of hemolytic transfusion reactions are triggered via several pathophysiological pathways like formation of anaphylatoxins, release of cytokines causing a systemic inflammatory response syndrome, activation of the kinin system, the intrinsic clotting cascade and fibrinolysis resulting in hypotension, disseminated intravascular coagulation, diffuse bleeding, and disruption of microcirculation leading to renal failure and shock 5

Symptoms and Complications

  • Symptoms of hemolytic transfusion reactions include fever, haemoglobinuria, and jaundice 2
  • Complications of hemolytic transfusion reactions can include bleeding, renal failure, and shock 2
  • In severe cases, hemolytic transfusion reactions can be fatal 6, 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Clinical presentation of haemolytic transfusion reactions.

Anaesthesia and intensive care, 1980

Research

Severe Acute Hemolytic Transfusion Reaction Treated with Ruxolitinib and Plasma Exchange.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 2021

Research

Hemolytic Transfusion Reactions.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 2008

Research

Hemolytic transfusion reaction: safeguards for practice.

Journal of perianesthesia nursing : official journal of the American Society of PeriAnesthesia Nurses, 2002

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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