What tests are included in initial blood work for a new pregnancy?

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Initial Blood Work for New Pregnancy

The initial blood work for a new pregnancy should include a complete blood count, blood type and Rh status, screening for infections including HIV, hepatitis B, syphilis, chlamydia, and gonorrhea, as well as rubella immunity status. 1, 2

Core Laboratory Tests for Initial Pregnancy Visit

Essential Blood Tests

  • Complete Blood Count (CBC) - Screens for anemia, which affects approximately 40% of pregnant women worldwide 3, 4

    • Hemoglobin <11.0 g/dL in first trimester indicates anemia
    • Mean corpuscular volume (MCV) helps differentiate types of anemia
  • Blood Type and Rh Status 2

    • Critical for identifying Rh-negative women who will need Rh immune globulin at 28 weeks
    • Includes ABO blood group and antibody screen
  • Infectious Disease Screening 2

    • HIV testing
    • Hepatitis B surface antigen (HBsAg)
    • Syphilis serology
    • Chlamydia and gonorrhea screening (especially for high-risk populations)
    • Hepatitis C antibody testing (for women with risk factors)
  • Immunity Status

    • Rubella antibody testing to determine immunity

Additional First Trimester Tests

  • Thyroid-stimulating hormone (TSH) - Consider measuring TSH levels to screen for thyroid dysfunction 1
  • Urinalysis - To screen for urinary tract infections and proteinuria 1

Genetic Screening Options

  • First trimester screening (11-14 weeks) 2

    • Nuchal translucency (NT) measurement
    • Pregnancy-associated plasma protein A (PAPP-A)
    • Human chorionic gonadotropin (hCG) or free beta-hCG
    • Detection rate of 75-80% for trisomy cases with 5% false-positive rate
  • Carrier screening based on family history, ethnicity, and risk factors 2

    • Cystic fibrosis and other carrier screening as indicated

Common Pitfalls and Important Considerations

Anemia Detection and Management

  • Anemia is the most common hematologic abnormality in pregnancy 3
  • Iron deficiency is the most common cause of anemia in pregnancy
  • Maternal anemia increases risk of transfusion at delivery and is associated with adverse fetal outcomes
  • The 5th percentile for hemoglobin in iron-replete women is approximately 110 g/L in the first trimester 5

Timing of Tests

  • Pregnancy dating should be calculated using the first day of the last menstrual period and/or an early-dating ultrasound 2
  • First trimester ultrasound (ideally between 7-14 weeks) provides the most accurate dating
  • Pregnancy tests can detect hCG as early as 3-4 days after implantation, with 98% accuracy by the time of the expected period 6

Special Considerations

  • For women with multiple gestation, more frequent ultrasound examinations are required than for singleton pregnancies 2
  • Women with history of preeclampsia should undergo first-trimester screening to assess risk of early-onset preeclampsia 1
  • For women at high risk of preterm preeclampsia, aspirin prophylaxis should begin before 16 weeks' gestation 1

By ensuring comprehensive initial blood work, healthcare providers can identify risk factors early and implement appropriate interventions to optimize maternal and fetal outcomes.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Pregnancy Care and Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Iron Deficiency Anemia in Pregnancy.

Obstetrics and gynecology, 2021

Research

Laboratory approach to investigation of anemia in pregnancy.

International journal of laboratory hematology, 2021

Research

Pregnancy tests: a review.

Human reproduction (Oxford, England), 1992

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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