What lab tests are indicated for low libido?

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Laboratory Evaluation for Low Sex Drive

For patients with low libido, a morning serum testosterone level should be measured as the primary laboratory test, especially in men with symptoms or signs of hypogonadism. 1

Primary Laboratory Tests

For Men:

  • Morning total testosterone level - Must be collected in the morning when levels are highest 1
  • Free testosterone calculation - Using total testosterone, sex hormone binding globulin (SHBG), and albumin concentrations 1, 2
  • Luteinizing hormone (LH) and follicle-stimulating hormone (FSH) - To determine if hypogonadism is primary or secondary 1

For Women:

  • Free testosterone index - Low free testosterone is significantly associated with decreased sexual desire in women 3
  • Sex hormone binding globulin (SHBG) - To calculate free testosterone index 3

Additional Laboratory Workup

Cardiovascular Risk Assessment

  • Lipid panel - Low HDL cholesterol is associated with sexual dysfunction 1
  • Fasting glucose/HbA1c - Diabetes is strongly associated with sexual dysfunction 1
  • Blood pressure measurement - Hypertension contributes to sexual dysfunction 1

Other Hormonal Tests

  • Thyroid function tests (TSH, free T4) - Thyroid disorders can affect libido 4
  • Prolactin level - Hyperprolactinemia can decrease sexual desire 4
  • Estradiol - Particularly important in women 3

Clinical Considerations

Men-Specific Considerations

  • Testosterone levels decline with age but symptoms don't always correlate with specific levels 4
  • Low testosterone is associated with decreased sexual desire, reduced nocturnal erections, and reduced ejaculate volume 4
  • 36% of men seeking consultation for sexual dysfunction have hypogonadism 1
  • Low testosterone is associated with increased all-cause and cardiovascular mortality 1

Women-Specific Considerations

  • Free testosterone index correlates with sexual thoughts and "need for sex" in women 3
  • HSDD (Hypoactive Sexual Desire Disorder) affects approximately 30% of women 5
  • The relationship between testosterone levels and sexual function in women is complex and may be confounded by limitations of assays at low concentrations 4

Common Pitfalls to Avoid

  • Timing errors: Testosterone should be measured in the morning when levels are highest 1
  • Using total testosterone alone: Free testosterone is the metabolically active fraction and may be more clinically relevant 2
  • Overlooking cardiovascular risk: ED and low libido may be early markers of cardiovascular disease 1
  • Ignoring sleep disorders: Obstructive sleep apnea can contribute to low testosterone and should be screened for in at-risk patients 1, 6
  • Missing psychological factors: Depression strongly correlates with sexual dysfunction, especially in women with diabetes 4

Monitoring Recommendations

For patients starting testosterone therapy:

  • Monitor total testosterone levels (target: 450-600 ng/dL)
  • Monitor hematocrit (discontinue if exceeds 54%)
  • Monitor PSA (consider biopsy if >4.0 ng/mL or increases >1.0 ng/mL/year) 6

By following this comprehensive laboratory evaluation, clinicians can identify the underlying causes of low libido and develop appropriate treatment strategies to improve sexual function and quality of life.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Testosterone Supplementation in Patients with Obstructive Sleep Apnea

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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