How to evaluate and manage patients with low total testosterone levels?

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Last updated: September 29, 2025View editorial policy

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Evaluation and Management of Low Total Testosterone Levels

The evaluation of low total testosterone requires confirmation with two morning measurements below 350 ng/dL along with consistent symptoms, followed by appropriate laboratory testing to determine the cause before initiating testosterone replacement therapy. 1

Diagnostic Approach

Initial Evaluation

  • Measure morning total testosterone levels on at least two separate days 1, 2
  • Confirm low testosterone when:
    • Total testosterone consistently below 350 ng/dL
    • Patient exhibits symptoms of testosterone deficiency
  • Symptoms suggesting testosterone deficiency:
    • Sexual: Decreased libido, erectile dysfunction
    • Non-sexual: Fatigue, depressed mood, irritability, decreased energy, reduced muscle mass and strength

Laboratory Evaluation

  • Essential laboratory tests:
    • Morning total testosterone (between 8-10 AM) 1
    • Free testosterone (particularly when total T is borderline or SHBG abnormality is suspected) 1, 2
    • Luteinizing hormone (LH) and follicle-stimulating hormone (FSH) 1
    • Complete blood count (CBC) 1
    • Prostate-specific antigen (PSA) in men over 40 1

Interpreting Results

  • Total testosterone thresholds for treatment consideration 1:
    • <230 ng/dL: Usually benefits from treatment
    • 231-346 ng/dL: Consider 4-6 month trial if symptomatic
    • 350 ng/dL: Treatment generally not indicated

  • Distinguish between primary and secondary hypogonadism:
    • Primary: Low testosterone with elevated LH/FSH (testicular failure)
    • Secondary: Low testosterone with low/normal LH/FSH (hypothalamic-pituitary disorder)

Management Approach

Non-Pharmacological Interventions

  • Lifestyle modifications should be recommended for all patients 1:
    • Weight loss for overweight/obese men
    • Regular aerobic exercise
    • Adequate sleep hygiene
    • Stress reduction techniques
    • Healthy diet
    • Moderate alcohol consumption
    • Smoking cessation

Pharmacological Treatment

  • Testosterone replacement therapy (TRT) options:
  1. Injectable testosterone 1, 3:

    • Testosterone enanthate/cypionate: 50-100 mg weekly or 200-400 mg every 2-4 weeks
    • Administer deeply into gluteal muscle
    • Most cost-effective option but requires injections
  2. Transdermal formulations 1:

    • Testosterone gel 1%: 50-100 mg daily
    • Convenient daily application but risk of transfer to others
  3. Testosterone pellets 1:

    • Long-acting option (3-6 months)
    • Requires procedural insertion

Contraindications to TRT

  • Active prostate or breast cancer 1, 2, 4
  • Hematocrit >50% 1, 3
  • Severe untreated sleep apnea 1
  • Uncontrolled heart failure 1, 3
  • Recent cardiovascular events (within 3-6 months) 1, 4
  • PSA >4.0 ng/mL or >3.0 ng/mL in high-risk men 4
  • Severe lower urinary tract symptoms 4
  • Desire for fertility in the near term 4

Monitoring and Follow-up

Monitoring Schedule

  • Baseline: Total testosterone, hematocrit, PSA, digital rectal examination 1
  • Follow-up:
    • 3-6 months after starting therapy: Total testosterone, hematocrit, PSA 1
    • Annually thereafter if stable 1

Target Parameters and Action Thresholds

  • Total testosterone: Target 450-600 ng/dL 1
  • Hematocrit: Discontinue if >54% 1
  • PSA thresholds requiring action 1:
    • 4.0 ng/mL: Consider prostate biopsy

    • Increase >1.0 ng/mL in any year: Perform biopsy
    • Increase 0.7-0.9 ng/mL in one year: Repeat PSA in 3-6 months

Benefits and Risks

Benefits of TRT

  • Improved sexual function (libido and erectile function) 1
  • Increased energy levels and reduced fatigue 1
  • Increased muscle mass and strength 1
  • Improved bone mineral density 1
  • Enhanced sense of well-being 1

Potential Risks

  • Erythrocytosis (increased hematocrit) 1, 3
  • Potential for sleep apnea exacerbation, especially with higher doses 1
  • Skin reactions (more common with patches than gels) 1
  • Decreased fertility 1
  • Potential cardiovascular effects (evidence is inconclusive) 1, 3
  • Risk of abuse at doses higher than recommended 3

Common Pitfalls to Avoid

  • Diagnosing testosterone deficiency based on a single measurement
  • Initiating treatment without confirming low testosterone with repeat testing
  • Failing to evaluate for underlying causes of hypogonadism
  • Not monitoring hematocrit, which can increase significantly during treatment
  • Overlooking contraindications before starting therapy
  • Neglecting to inform patients about potential fertility impacts

References

Guideline

Testosterone Replacement Therapy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Testosterone Therapy in Men With Hypogonadism: An Endocrine Society Clinical Practice Guideline.

The Journal of clinical endocrinology and metabolism, 2018

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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