What is the recommended dosing regimen for Prolia (denosumab) injection?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: October 8, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Recommended Dosing Regimen for Prolia (Denosumab) Injection

The recommended dose of Prolia (denosumab) is 60 mg administered as a single subcutaneous injection once every 6 months. 1

Administration Details

  • Prolia should be administered by a healthcare professional via subcutaneous injection in the upper arm, upper thigh, or abdomen 1
  • Prior to administration, Prolia may be removed from the refrigerator and brought to room temperature (up to 25°C/77°F) by standing in the original container for 15-30 minutes 1
  • Do not warm Prolia in any other way 1
  • Visually inspect the solution for particulate matter and discoloration before administration - it should be clear, colorless to pale yellow 1

Important Pre-Administration Requirements

  • Pregnancy testing must be performed in all females of reproductive potential prior to administration of Prolia 1
  • For patients with advanced chronic kidney disease (eGFR < 30 mL/min/1.73 m²), evaluate for chronic kidney disease mineral and bone disorder (CKD-MBD) with intact parathyroid hormone, serum calcium, and vitamin D levels 1
  • All patients should receive calcium 1000 mg daily and at least 400 IU vitamin D daily as supplementation 1

Clinical Efficacy

  • Denosumab has demonstrated significant increases in bone mineral density (BMD) in postmenopausal women with osteoporosis 2
  • In clinical studies, denosumab treatment resulted in BMD increases of 4.2% at 12 months, 7.5% at 24 months, and 8.8% at 36 months in the lumbar spine 2
  • The ABCSG-18 trial showed that denosumab significantly delayed time to occurrence of clinical fractures (HR, 0.5; 95% CI, 0.39 to 0.65; P < .001) 3

Missed Doses

  • If a dose of Prolia is missed, administer the injection as soon as the patient is available 1
  • Thereafter, schedule injections every 6 months from the date of the last injection 1

Advantages of Denosumab

  • The 6-month dosing interval may improve patient adherence compared to more frequently dosed medications 4, 5
  • Denosumab does not require renal dose adjustment, which may be advantageous for patients with reduced kidney function 6
  • Subcutaneous administration may be preferred by patients who have difficulty with oral medications 5, 7

Safety Considerations

  • Monitor for hypocalcemia, which is a listed contraindication 3
  • When denosumab is discontinued, there may be an increased risk of vertebral fractures 3
  • The European Calcified Tissue Society suggests using a bisphosphonate when stopping denosumab to reduce fracture risk 3

Clinical Pearls

  • Denosumab has a novel mechanism of action - it binds to receptor activator of nuclear factor κB ligand (RANKL) and inhibits bone resorption by inhibiting osteoclast formation, function, and survival 5
  • The convenience of subcutaneous administration every 6 months may contribute to better compliance with treatment recommendations 2, 4
  • Denosumab may be particularly useful for patients with gastrointestinal contraindications or side effects from oral bisphosphonates 7

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.