Is Congenital Adrenal Hyperplasia (CAH) included in newborn screening?

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Last updated: October 9, 2025View editorial policy

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Congenital Adrenal Hyperplasia (CAH) in Newborn Screening

Yes, Congenital Adrenal Hyperplasia (CAH) is included in the core panel of disorders recommended for newborn screening in the United States. 1

Evidence and Recommendations

CAH is one of the high-scoring conditions identified by the American College of Medical Genetics (ACMG) as appropriate for inclusion in newborn screening programs:

  • CAH was among the top scoring conditions in the ACMG evaluation system, following medium-chain acyl CoA dehydrogenase (MCAD) deficiency, congenital hypothyroidism (CH), phenylketonuria (PKU), biotinidase deficiency (BIOT), and sickle cell anemia (Hb SS) 1
  • It is officially designated as part of the core panel of disorders recommended for universal newborn screening 1
  • The screening is performed using tandem mass spectrometry (MS/MS) which measures 17α-hydroxyprogesterone (17-OHP) levels in dried blood spots 1

Screening Effectiveness and Limitations

The effectiveness of CAH screening varies by disease subtype:

  • Newborn screening is highly effective for detecting the salt-wasting (SW) form of CAH, which is the most severe and potentially life-threatening form 2
  • Studies have shown that all salt-wasting CAH patients would be detected using recommended cut-off limits for 17-OHP (30 nmol/l) 2
  • However, approximately one-third of children with the moderate (simple virilizing) form of CAH may be missed by screening, resulting in a significant false-negative rate 2, 3
  • False-positive rates remain relatively high compared to other newborn screening disorders, particularly in premature and low birth weight infants 3, 4

Screening Improvements

Several approaches have been implemented to improve CAH screening:

  • Some states have implemented two-tier screening protocols with second-tier steroid profiling to reduce false positives 3
  • Establishing normative reference levels based on birth weight or gestational age may help minimize false-positive rates, particularly in low birth weight newborns 4
  • Molecular analysis (CYP21A2 gene sequencing) has been found valuable as a confirmatory test for children with persistently elevated 17-OHP levels 5

Clinical Implications

Healthcare providers should be aware of important clinical considerations:

  • Despite inclusion in newborn screening, physicians should be cautioned that a negative newborn screen does not necessarily rule out classic CAH 3
  • Any patient with clinical concerns for CAH should receive immediate diagnostic testing regardless of screening results 3, 6
  • Early diagnosis through screening helps prevent life-threatening salt-wasting crises and allows for timely treatment to prevent permanent negative effects of androgen overproduction 2, 6

Conclusion

CAH screening is an established component of the recommended uniform screening panel, with particular effectiveness for detecting the most severe salt-wasting form. However, healthcare providers should be aware of its limitations, particularly for moderate forms of the disorder, and maintain clinical vigilance regardless of screening results.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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