What are the grades of neutropenia in chemotherapy?

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Last updated: October 17, 2025View editorial policy

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Grades of Neutropenia in Chemotherapy

Neutropenia in chemotherapy is graded based on the absolute neutrophil count (ANC), with Grade 4 neutropenia being the most severe and associated with highest risk of complications.

Neutropenia Grading System

  • Grade 1 Neutropenia: ANC 1500-2000/mm³ (mild neutropenia) 1
  • Grade 2 Neutropenia: ANC 1000-1500/mm³ (moderate neutropenia) 1
  • Grade 3 Neutropenia: ANC 500-1000/mm³ (severe neutropenia) 1
  • Grade 4 Neutropenia: ANC <500/mm³ (life-threatening neutropenia) 1, 2

Clinical Significance of Neutropenia Grades

Febrile Neutropenia

  • Febrile neutropenia is defined as an axillary temperature >38.5°C for >1 hour with an ANC <500/mm³ 1
  • Represents a medical emergency requiring prompt intervention 1
  • Mortality rates in febrile neutropenia range from 0-7% in standard chemotherapy regimens 1

Risk Assessment

  • High-risk regimens: Those with expected neutropenia rate >50%, such as 3-drug combinations including lenalidomide plus alkylating agents 3
  • Low-risk regimens: Combinations of novel agents with dexamethasone alone 3
  • Grade 4 neutropenia incidence >65% predicts higher risk for febrile neutropenia and other adverse outcomes 2

Management Based on Neutropenia Grade

Grade 3 Neutropenia (ANC 500-1000/mm³)

  • Monitor closely for fever and signs of infection 1
  • Consider dose modifications for subsequent chemotherapy cycles 1
  • For persistent Grade 3 neutropenia (>2 weeks), consider dose reduction or growth factor support 1

Grade 4 Neutropenia (ANC <500/mm³)

  • Hold chemotherapy until ANC recovery to ≥1000-1500/mm³ 1
  • Resume at reduced dose if neutropenia persists beyond expected recovery time 1
  • Consider granulocyte colony-stimulating factors (G-CSF) for management 3
  • Hospitalization and broad-spectrum antibiotics if febrile 1

G-CSF Prophylaxis Recommendations

Primary Prophylaxis (Before Neutropenia Occurs)

  • Recommended for high-risk chemotherapy regimens (>20% risk of febrile neutropenia) 3, 4
  • Consider for intermediate-risk regimens (10-20% risk) with additional risk factors 3
  • Standard dose: 5 μg/kg/day of G-CSF subcutaneously 24-72 hours after chemotherapy until ANC recovery 1
  • Pegfilgrastim (6 mg single dose) is equally effective as daily G-CSF 1, 5

Secondary Prophylaxis (After Neutropenia Episode)

  • Indicated when patients experience Grade 3/4 neutropenia with prior cycles 3
  • If ANC recovers to >1000/mm³, therapy can resume without dose modifications 3
  • If severe neutropenia persists, delay treatment until ANC >1000/mm³ and implement dose reductions 3

Special Considerations

  • G-CSF is contraindicated during chest radiotherapy due to increased complications and mortality 1
  • Pretreatment absolute monocyte count <370/mm³ and/or absolute neutrophil count <3500/mm³ may predict higher risk for febrile neutropenia 6
  • Neutropenia incidence varies by malignancy type: approximately 10% in breast cancer patients versus 40% in lymphoma patients receiving moderate/high-risk chemotherapy 4

Monitoring Recommendations

  • Regular complete blood counts with differential during chemotherapy 1
  • More frequent monitoring during expected nadir periods 1
  • Patient education regarding fever and infection symptoms requiring immediate medical attention 1

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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