What investigations are recommended for patients presenting with myalgia (muscle pain)?

Recommended Investigations for Myalgia

The diagnostic workup for patients presenting with myalgia should include a complete rheumatologic and neurologic history, physical examination with muscle strength assessment, and basic laboratory testing to evaluate muscle inflammation and exclude mimicking conditions. 1

Initial Laboratory Investigations

• Complete blood count to assess for anemia which may be present in inflammatory conditions 1, 2
• Inflammatory markers: ESR and CRP to evaluate inflammation (ESR >40 mm/h is common in inflammatory myalgias like polymyalgia rheumatica) 1, 3
• Muscle enzymes: Creatine kinase (CK), aldolase, transaminases (AST, ALT), and lactate dehydrogenase (LDH) to assess for muscle inflammation or damage 1, 2
• Thyroid function tests (TSH) to rule out hypothyroidism as a cause of myalgia 1, 4
• Basic metabolic panel: glucose, creatinine, electrolytes 1
• Liver function tests 1
• Bone profile including calcium and alkaline phosphatase 1
• Urinalysis to screen for myoglobinuria in cases of suspected rhabdomyolysis 1

Additional First-Line Testing

• Autoimmune serologies: Rheumatoid factor, anti-CCP antibodies, and ANA to exclude rheumatoid arthritis and other autoimmune conditions 1
• Vitamin D level as deficiency can cause or exacerbate myalgia 1, 4
• Protein electrophoresis to evaluate for paraproteinemia 1

Second-Line Investigations (Based on Clinical Suspicion)

• Specific autoantibody testing:

  • Myositis-specific antibodies if inflammatory myopathy is suspected 1
  • Anti-acetylcholine receptor and anti-striated muscle antibodies if myasthenia gravis is suspected 1
  • ANCA if vasculitis is suspected 1

• Imaging studies:

  • MRI of affected muscle groups to assess for inflammation, edema, or structural abnormalities 1
  • Ultrasound of joints if arthritis is suspected 1
  • Plain radiographs to exclude metastases or evaluate joint damage if arthritis is suspected 1
  • Chest radiograph to exclude underlying malignancy or sarcoidosis 1

• Electrophysiologic studies:

  • Electromyography (EMG) to differentiate between myopathic, neurogenic, or normal patterns 1, 5
  • Nerve conduction studies to evaluate for peripheral neuropathy 5

• Muscle biopsy when diagnosis remains uncertain despite other investigations, particularly when inflammatory myopathy is suspected 1, 2

Specialized Testing Based on Clinical Context

• Cardiac evaluation if myocarditis is suspected:

  • Troponin (troponin I is more specific than troponin T in skeletal muscle disease) 1
  • Electrocardiogram 1
  • Echocardiogram or cardiac MRI if abnormalities are detected 1

• Infectious disease testing if clinically indicated:

  • Tuberculosis testing 1
  • Viral hepatitis serologies 1

Investigation Algorithm Based on Presentation

For Patients with Proximal Muscle Pain Without Weakness

1. Basic laboratory tests (CBC, ESR, CRP, CK, TSH)
2. If ESR/CRP elevated but CK normal, consider polymyalgia rheumatica, especially in patients >50 years 3, 6
3. If CK elevated, proceed with myositis workup including autoantibodies, MRI, and EMG 1

For Patients with Muscle Pain and Weakness

1. Complete laboratory panel including muscle enzymes
2. If CK elevated (≥3× ULN), urgent evaluation for myositis with MRI, EMG, and possible biopsy 1
3. Cardiac evaluation to rule out myocarditis 1
4. Consider autoantibody testing for myositis and myasthenia gravis 1

For Patients with Normal Initial Testing

1. Consider fibromyalgia or myofascial pain syndrome 4
2. Evaluate for mechanical/structural causes (scoliosis, joint hypomobility) 4
3. Consider metabolic factors (iron deficiency, vitamin D deficiency) 4

Important Considerations

• Muscle weakness is more typical of myositis than pain alone 1
• Normal CK levels generally differentiate polymyalgia-like syndromes from true myositis 1
• Some patients with polymyalgia rheumatica may have ESR <40 mm/h (about 20% of cases), particularly men and younger patients 6
• Early recognition of myositis is critical, especially if associated with myocarditis or bulbar symptoms, as it can be life-threatening 1