What is the diagnostic yield for Endobronchial Ultrasound-guided Transbronchial Needle Aspiration (EBUS-TBNA)?

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Last updated: October 23, 2025View editorial policy

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Diagnostic Yield of EBUS-TBNA

EBUS-TBNA has a pooled diagnostic accuracy of approximately 78-79% across various conditions, with higher yields for certain pathologies like tuberculosis (80-85%) and lower yields for lymphoma (69%).

Overall Diagnostic Accuracy

  • EBUS-TBNA provides safe and minimally invasive access to mediastinal and hilar lymph nodes with a pooled diagnostic accuracy of 79.1% across various conditions 1
  • A systematic review and meta-analysis including 15 studies with 553 patients showed diagnostic yields ranging from 54% to 93%, with a pooled accuracy of 79% (95% CI, 71-86) 1
  • When additional studies were included (total of 25 studies with 1,126 patients), the pooled diagnostic accuracy was 78.2% (95% CI, 75.6-80.4) 1

Disease-Specific Diagnostic Yields

Sarcoidosis

  • For suspected sarcoidosis with mediastinal/hilar adenopathy, EBUS-TBNA is recommended with Grade 1C evidence 1
  • EBUS-TBNA had a yield of 74.5% for sarcoidosis, which was significantly better than conventional TBNA (48.4%, p=0.004) and endobronchial biopsy (36.3%, p<0.0001) 1
  • Combining EBUS-TBNA with transbronchial lung biopsy (TBLB) significantly enhances the diagnostic yield 1

Tuberculosis

  • For suspected tuberculosis with mediastinal/hilar adenopathy, EBUS-TBNA is recommended with Grade 1C evidence 1
  • Diagnostic yield for TB ranges from 79.2% to 84.8% across studies 1
  • In TB-endemic populations, EBUS-TBNA achieved a high yield of 84.8% even during operators' early experience 1

Lymphoma

  • EBUS-TBNA has a lower diagnostic yield for lymphoma compared to other conditions, with a pooled diagnostic accuracy of 68.7% (95% CI, 61.9-75.5) 1
  • Diagnostic yield varies significantly between studies (38-91%), with higher yields for relapsed lymphoma compared to de novo lymphoma 1
  • For suspected new cases of lymphoma, especially Hodgkin lymphoma, the diagnostic yield can be as low as 25-61% 2
  • The diagnostic accuracy for lymphoma is affected by the need for histologic architecture assessment and immunophenotyping 1

Factors Affecting Diagnostic Yield

  • Lymph node characteristics: Nodes ≤5mm in size and those in paratracheal locations have lower sampling accuracy 3
  • Needle size: 19G, 21G, and 22G needles show similarly high diagnostic sensitivity (93%, 87.6%, and 85% respectively) 4
  • Number of passes: Optimal diagnostic values can be obtained after a minimum of three passes per sampling site 1
    • Sample adequacy reaches 90.1% after the first pass, 98.1% after two passes, and 100% after three passes 1
    • Sensitivity for differentiating malignant from benign lymph nodes increases from 69.8% with one pass to 95.3% with three passes 1
  • Rapid on-site evaluation (ROSE): No significant difference in diagnostic yield between procedures with ROSE (80.1%) and without ROSE (81.3%) 1
  • Complementary techniques: Combining EBUS-TBNA with transbronchial forceps biopsy can improve diagnostic yield from 73% to 86%, particularly for granulomatous and lymphoproliferative disorders 5

Comparison to Other Techniques

  • EBUS-TBNA (74.5%) shows superior diagnostic yield compared to conventional TBNA (48.4%) and endobronchial biopsy (36.3%) 1
  • For lung cancer and mediastinal staging, EBUS-TBNA demonstrates high sensitivity (86.1-88.3%) and specificity (96.4-100%) 6, 3
  • When sampling is limited to enlarged right paratracheal and subcarinal lymph nodes, EBUS-TBNA yield (74.5%) is comparable to transbronchial lung biopsy (69.9%) 1

Practical Considerations

  • In the absence of rapid on-site evaluation, a minimum of 3 separate needle passes per sampling site is recommended for optimal diagnostic yield 1
  • For molecular and immunohistochemical testing, 19G needles may provide better sample adequacy 4
  • Operator experience affects diagnostic yield, with EBUS-STAT scores showing significant differences between beginners (31.1), intermediate (74.9), and experienced (93.6) bronchoscopists 1
  • Airway distortion and lymph node calcification are associated with less accurate EBUS-TBNA results 3

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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