What labs should be ordered to evaluate low testosterone?

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Laboratory Tests for Evaluating Low Testosterone

The diagnosis of low testosterone requires two early morning total testosterone measurements below 300 ng/dL on separate occasions, combined with symptoms and/or signs of testosterone deficiency. 1

Initial Laboratory Evaluation

  • Measure total testosterone in an early morning fashion (8-10 AM) when levels are highest due to diurnal variation 1
  • Confirm with a second early morning total testosterone measurement on a separate occasion, preferably using the same laboratory with the same method/instrumentation 1
  • Total testosterone below 300 ng/dL is the recommended cut-off to support the diagnosis of low testosterone 1

Additional Required Testing

  • Measure serum luteinizing hormone (LH) levels to establish the etiology of testosterone deficiency (primary vs. secondary hypogonadism) 1
  • Measure serum prolactin levels in patients with low testosterone combined with low or low/normal LH levels 1
  • Consider measuring free testosterone by equilibrium dialysis or calculation when total testosterone is near the lower limit of normal or when SHBG abnormality is suspected 2, 3

Special Considerations for Free Testosterone

  • Total testosterone alone may not be sufficient to exclude hypogonadism, particularly in men over 60 years old or those with conditions that alter SHBG 4
  • Total testosterone must exceed 350-400 ng/dL to reliably predict normal free testosterone 5
  • In patients with normal SHBG but reduced total testosterone, free testosterone measurement is essential to determine if true testosterone deficiency exists 2

Clinical Context for Testing

  • Document symptoms associated with low testosterone: reduced energy, reduced endurance, diminished work/physical performance, fatigue, visual field changes, anosmia, depression, reduced motivation, poor concentration, impaired memory, irritability, infertility, reduced sex drive, and erectile dysfunction 1
  • Conduct targeted physical exam: body habitus, virilization status, BMI/waist circumference, gynecomastia, testicular evaluation, varicocele presence, and prostate assessment 1

High-Risk Populations

Consider measuring total testosterone even in asymptomatic patients with:

  • Unexplained anemia 1
  • Bone density loss 1
  • Diabetes 1
  • Exposure to chemotherapy or testicular radiation 1
  • HIV/AIDS 1
  • Chronic narcotic or corticosteroid use 1
  • Male infertility 1
  • Pituitary dysfunction 1

Common Pitfalls

  • Validated questionnaires are not recommended for identifying candidates for testosterone therapy due to variable specificity and sensitivity 1
  • Single testosterone measurements are insufficient; confirmation with a second measurement is required 1
  • Normal total testosterone does not exclude hypogonadism in men with elevated SHBG, particularly in older men 6, 4
  • Morning measurements are essential due to diurnal variation in testosterone levels 1
  • Free androgen index correlates poorly with calculated free testosterone and may overestimate free testosterone at low SHBG concentrations 7

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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