What is the ratio of Adrenocorticotropic hormone (ACTH) to cortisol in secondary adrenal insufficiency?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: October 26, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

ACTH to Cortisol Ratio in Secondary Adrenal Insufficiency

In secondary adrenal insufficiency, the ACTH to cortisol ratio is typically low, with low or low-normal ACTH levels and low or intermediate cortisol levels (5-10 µg/dL). This pattern of low ACTH with low cortisol distinguishes secondary adrenal insufficiency from primary adrenal insufficiency, which presents with high ACTH and low cortisol levels. 1

Diagnostic Patterns in Secondary Adrenal Insufficiency

  • Secondary adrenal insufficiency is characterized by low or low-normal ACTH levels with low or intermediate cortisol levels, reflecting the underlying pituitary or hypothalamic disorder 2, 1
  • Morning cortisol levels are typically in the range of 5-10 µg/dL, but a single measurement is insufficient for definitive diagnosis 2, 1
  • Unlike primary adrenal insufficiency (where ACTH is elevated), secondary adrenal insufficiency shows inadequate ACTH production leading to cortisol deficiency 3, 1
  • The 250-μg ACTH stimulation test is the recommended diagnostic test for evaluating adrenal insufficiency in patients with suspected hypopituitarism 2, 3

Laboratory Findings in Secondary vs. Primary Adrenal Insufficiency

  • In secondary adrenal insufficiency:

    • ACTH levels: Low or low-normal
    • Cortisol levels: Low or intermediate (5-10 µg/dL)
    • DHEAS levels: Low or low-normal 1
  • In primary adrenal insufficiency:

    • ACTH levels: High (compensatory increase)
    • Cortisol levels: Very low (<5 µg/dL)
    • DHEAS levels: Low 1

ACTH Stimulation Testing

  • The high-dose (250-μg) ACTH stimulation test is recommended over the low-dose (1-μg) test due to comparable diagnostic accuracy and easier practical administration 3
  • In secondary adrenal insufficiency, the cortisol secretion rate (CSR) in response to ACTH is significantly reduced compared to healthy controls 4
  • Maximal cortisol secretion rate (CSRmax) is approximately 0.17 ± 0.09 nM/s in secondary adrenal insufficiency versus 0.46 ± 0.14 nM/s in healthy controls 4
  • The ACTH stimulation test helps differentiate between primary and secondary adrenal insufficiency, as patients with secondary adrenal insufficiency typically have normal aldosterone responses but subnormal cortisol responses 5

Clinical Implications

  • Basal cortisol levels can be useful in predicting adrenal insufficiency:
    • Cortisol ≥450 nmol/L has a 98.7% negative predictive value to rule out adrenal insufficiency
    • Cortisol ≤100 nmol/L has a 93.2% positive predictive value to rule in adrenal insufficiency 6
  • Patients with secondary adrenal insufficiency continue to secrete aldosterone in response to renin, unlike those with primary adrenal insufficiency who are deficient in both cortisol and aldosterone 3
  • Secondary adrenal insufficiency carries significant mortality risk, with risk ratios of 2.19 for men and 2.86 for women 3

Management Considerations

  • Treatment involves supplemental glucocorticoids (e.g., hydrocortisone 15-25 mg daily or prednisone 3-5 mg daily) 1
  • Unlike primary adrenal insufficiency, mineralocorticoid replacement (fludrocortisone) is not required in secondary adrenal insufficiency since aldosterone secretion remains intact 3, 1
  • Patients should be educated about stress dosing during illness or surgery to prevent adrenal crisis 7
  • Perioperative management requires increased glucocorticoid dosing based on the severity of surgical stress 3

Pitfalls and Caveats

  • A single morning cortisol measurement is insufficient for diagnosis; ACTH stimulation testing is required for confirmation 2, 8
  • When starting both glucocorticoid and thyroid replacement in hypopituitarism, always start glucocorticoids first to prevent precipitating adrenal crisis 7
  • Adrenal crisis is a life-threatening emergency that can occur in patients with adrenal insufficiency who have inadequate glucocorticoid therapy during acute illness or physical stress 1, 8
  • Inhaled corticosteroid therapy can cause suppression of the hypothalamo-pituitary-adrenal axis, potentially leading to secondary adrenal insufficiency 3

References

Guideline

Diagnosis of Adrenal Insufficiency in Hypopituitarism

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

DIAGNOSTIC ACCURACY OF BASAL CORTISOL LEVEL TO PREDICT ADRENAL INSUFFICIENCY IN COSYNTROPIN TESTING: RESULTS FROM AN OBSERVATIONAL COHORT STUDY WITH 804 PATIENTS.

Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists, 2017

Guideline

Management of Hypercortisolism with Adrenal Insufficiency Symptoms

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Diagnosis and management of adrenal insufficiency.

Clinical medicine (London, England), 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.