When should macrophage activation syndrome (MAS) be suspected in a case of Dengue?

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Macrophage Activation Syndrome in Dengue: When to Suspect

Macrophage Activation Syndrome (MAS) should be suspected in dengue patients with persistent high fever beyond 7 days, worsening cytopenias, hepatosplenomegaly, hyperferritinemia (>10,000 ng/ml), and development of multiorgan dysfunction despite resolution of the critical phase. 1, 2, 3

Clinical Indicators for MAS in Dengue

  • Persistent high fever spikes that continue beyond the expected resolution of dengue's critical phase 1, 3
  • Development of hepatosplenomegaly during or after the critical phase of dengue 2, 3
  • Worsening cytopenias (thrombocytopenia, neutropenia, anemia) despite resolution of the critical phase 1, 3
  • Abnormal liver function tests with features of liver failure 2
  • Coagulopathy that persists or worsens after critical phase 2
  • Neurological complications not typical for uncomplicated dengue 2

Laboratory Parameters to Monitor

  • Serum ferritin levels >3,000 ng/ml (particularly >10,000 ng/ml is highly suspicious for MAS) 4, 3
  • Elevated soluble CD163 (sCD163) levels, which can differentiate severe dengue from dengue fever 5
  • Elevated triglyceride levels (>265 mg/dL) 3
  • Declining fibrinogen levels 2
  • Persistently elevated or worsening liver enzymes 2
  • Prolonged prothrombin time/international normalized ratio (PT/INR) 2
  • High erythrocyte sedimentation rate (ESR) despite improvement in other dengue parameters 2

Diagnostic Approach

  • Consider bone marrow evaluation in suspected cases, which can confirm hemophagocytosis 2, 3
  • Apply HLH-2004 diagnostic criteria when suspecting MAS in dengue patients 3
  • Monitor for multiorgan dysfunction, which commonly occurs concurrently with MAS in dengue 2
  • Differentiate from prolonged dengue by looking for the constellation of persistent fever, cytopenias, and extremely elevated ferritin levels 1, 3

High-Risk Scenarios

  • Patients with prolonged critical phase of dengue 1
  • Cases with unusual clinical features like hepatosplenomegaly developing during dengue infection 3
  • Patients showing signs of autoimmune response during dengue infection 6
  • Dengue patients with multiorgan dysfunction not responding to standard supportive care 1

Management Considerations

  • Early recognition and prompt treatment are crucial for good outcomes 3
  • Consider immunosuppressive therapy with corticosteroids (e.g., dexamethasone 10 mg/BSA/day) for confirmed cases 3
  • Intravenous immunoglobulin (IVIG) has shown good results in pediatric dengue-associated HLH/MAS 1, 2
  • Monitor response to therapy with serial ferritin levels and improvement in cytopenias 3

Pitfalls and Caveats

  • MAS can be easily missed as its symptoms may be attributed to severe dengue itself 1
  • Delay in diagnosis and treatment significantly increases mortality 3
  • The surge of pro-inflammatory cytokines in dengue-associated MAS and multiorgan dysfunction may indicate they are part of a related inflammatory spectrum 1
  • Laboratory parameters of MAS may overlap with those of severe dengue, making diagnosis challenging 5

References

Research

Dengue infection associated hemophagocytic syndrome: Therapeutic interventions and outcome.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2015

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Macrophage Activation Syndrome-Associated Markers in Severe Dengue.

International journal of medical sciences, 2016

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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