What is the calculation for dilution of norepinephrine (Levophed) in D5W (Dextrose 5% in Water)?

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Norepinephrine (Levophed) Dilution in D5W

The standard dilution for norepinephrine (Levophed) in D5W is to add 4 mg of norepinephrine to 250 mL of D5W to yield a concentration of 16 μg/mL. 1

Standard Dilution Methods

  • For adult patients, the FDA-approved method is to add 4 mg (4 mL of a 1 mg/mL solution) of norepinephrine to 250 mL of D5W, resulting in a concentration of 16 μg/mL 1
  • Alternative dilution: Add 1 mg (1 mL) of 1:1000 dilution of norepinephrine to 250 mL of D5W to yield a concentration of 4.0 μg/mL 2
  • For anaphylaxis situations requiring continuous infusion, an alternative 1:100,000 solution can be prepared by adding 1 mg (1 mL) of norepinephrine in 100 mL of saline, administered at an initial rate of 30-100 mL/h (5-15 μg/min) 2

Administration Guidelines

  • Norepinephrine should be administered as a continuous infusion through a large vein, preferably via central venous access 1
  • Initial dosing typically starts at 2-3 mL/min (8-12 μg/min) of the standard 16 μg/mL solution, then titrated to maintain blood pressure 1
  • Average maintenance dose ranges from 0.5-1 mL/min (2-4 μg/min) of the standard solution 1
  • For peripheral administration (when central access is unavailable), more dilute solutions may be preferred to reduce the risk of extravasation 3

Important Considerations

  • D5W is the preferred diluent for norepinephrine as it provides protection against significant loss of potency due to oxidation 1
  • Administration in saline solution alone is not recommended by the FDA label 1
  • Norepinephrine solutions (4 μg/mL and 16 μg/mL) in D5W are chemically stable for up to seven days at room temperature under ambient light 4
  • Monitor for potential side effects including hypertension, arrhythmias, and tissue ischemia 3

Pediatric Considerations

  • For pediatric patients, the "rule of 6" can be used: 0.6 × body weight (kg) = number of milligrams diluted to total 100 mL of saline; then 1 mL/h delivers 0.1 μg/kg/min 2, 3
  • Pediatric dosing typically ranges from 0.1-1.0 μg/kg per minute, starting at the lowest dose and titrating to desired clinical effect 3

Precautions

  • Be aware that severe hyponatremia and seizures have been reported with peripheral infusion of dilute norepinephrine (4 μg/mL) in D5W 5
  • Ensure adequate volume resuscitation before and during norepinephrine administration 3
  • Avoid abrupt withdrawal; infusions should be reduced gradually 1
  • Do not mix norepinephrine with sodium bicarbonate or other alkaline solutions in the IV line, as they can inactivate the medication 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Norepinephrine Drip Administration Protocol

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Stability of norepinephrine infusions prepared in dextrose and normal saline solutions.

Canadian journal of anaesthesia = Journal canadien d'anesthesie, 2008

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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