Is it safe to perform a Pap (Papanicolaou) test during pregnancy?

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Safety of Pap Testing During Pregnancy

Yes, it is safe to perform a Pap test during pregnancy, and pregnant women should be screened at the same frequency as non-pregnant women. 1, 2

When to Perform Pap Testing in Pregnancy

  • A Pap smear should be obtained at the first prenatal visit if none has been documented during the preceding year. 1
  • Pregnant women should follow the same screening frequency as non-pregnant women, making pregnancy an excellent opportunity for cervical cancer screening. 1, 2
  • If a woman is menstruating, postpone the conventional cytology Pap test and advise her to have it at the earliest opportunity. 1, 2

Safe Collection Technique in Pregnancy

Use a swab and an Ayre's spatula for obtaining Pap tests in pregnant women, but do NOT use cytobrushes. 1, 2

  • Cytobrushes are contraindicated in pregnancy because they may disrupt the mucous plug. 2
  • The presence of mucopurulent discharge should not delay the Pap test—simply remove the discharge carefully with a saline-soaked cotton swab before obtaining the specimen. 1, 2
  • Liquid-based cytology is an acceptable alternative to conventional Pap tests with similar test-performance characteristics. 1

Evidence Supporting Safety

Research demonstrates that Pap testing during pregnancy is safe, necessary, and acceptable:

  • A study of 954 pregnant women found that cervical Pap smear testing is safe and acceptable for routine prenatal care, with abnormal epithelial changes detected in 9.3% of pregnant women (similar to non-pregnant controls). 3
  • The prevalence of squamous intraepithelial lesions (SIL) was actually lowest in pregnant women (0.94%) compared to non-pregnant controls (2.75%) and gynecological patients (6.23%). 3
  • A prospective study of 596 pregnant women showed a low prevalence (0.8%) of premalignant lesions, with only 1.5% unsatisfactory smears, demonstrating the feasibility of opportunistic screening during pregnancy. 4

Management Considerations

  • Management recommendations for abnormal Pap smears differ for pregnant women compared to non-pregnant women. 1, 2
  • Pregnant adolescents with minor Pap abnormalities may be followed rather than referred for immediate colposcopy due to high rates of HPV regression. 5
  • Postponing colposcopy in pregnant, reproductive-age women with minor Pap changes until after delivery is now acceptable. 5
  • Women with external genital warts do not need Pap tests more frequently than women without warts. 1, 2

Important Caveats

Do not skip Pap testing during pregnancy assuming it can wait until postpartum. Research shows that 4.9% of women with normal prenatal Pap smears had abnormal postpartum results, and Pap test results during pregnancy may not be accurate due to hormonal influences. 6, 7 However, this supports performing the test during pregnancy rather than avoiding it, as pregnancy provides a critical screening opportunity that should not be missed. 1, 4

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Cervical Cancer Screening in Pregnant Women

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

[Papanicolaou test in pregnancy].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 2000

Research

Abnormal Pap smear among pregnant women - Feasibility of opportunistic cervical screening.

European journal of obstetrics & gynecology and reproductive biology: X, 2023

Research

Caring for women with abnormal papanicolaou tests during pregnancy.

Journal of midwifery & women's health, 2009

Research

The postpartum Papanicolaou smear.

The Journal of the American Board of Family Practice, 1989

Research

Comparing Papanicolaou test results obtained during pregnancy and post-partum.

The journal of obstetrics and gynaecology research, 2017

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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