When to perform a biopsy on a thyroid nodule?

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Last updated: March 28, 2025 • View editorial policy

From the Guidelines

Thyroid nodules should be biopsied based on their size, ultrasound characteristics, and patient risk factors, with fine needle aspiration (FNA) biopsy generally recommended for nodules ≥1 cm with suspicious ultrasound features. The decision to perform a biopsy on a thyroid nodule should consider several factors, including the nodule's size, ultrasound characteristics, and patient risk factors, as outlined in the studies 1, 2, 3.

Key Considerations

  • Nodules ≥1 cm with suspicious ultrasound features, such as irregular margins, microcalcifications, hypoechogenicity, or a taller-than-wide shape, should be biopsied.
  • Nodules <1 cm may also require biopsy if there are clinical or ultrasonographic suspicions of malignancy, such as a history of head and neck irradiation, family history of thyroid cancer, or presence of cervical adenopathy.
  • Purely cystic nodules rarely require biopsy, as they are unlikely to be malignant.
  • Patient risk factors, such as history of radiation exposure, family history of thyroid cancer, or concerning symptoms like hoarseness or lymphadenopathy, may lower the threshold for biopsy.
  • TSH levels should also be considered, as elevated TSH slightly increases cancer risk.

Diagnostic Approach

  • Fine needle aspiration cytology (FNAC) is a sensitive and specific diagnostic tool for differentiating between benign and malignant nodules, although it has limitations, such as inadequate samples and follicular neoplasia 2.
  • In cases of inadequate samples, FNAC should be repeated, while follicular neoplasia with normal TSH and a "cold" appearance on thyroid scan may require surgical consideration.
  • Measurement of serum calcitonin is a reliable tool for diagnosing medullary thyroid cancer and should be an integral part of the diagnostic evaluation of thyroid nodules 1.

Management

  • The initial treatment of differentiated thyroid carcinoma (DTC) should always be preceded by careful exploration of the neck by ultrasound to assess the status of lymph node chains 2.
  • Total or near-total thyroidectomy is generally recommended for DTC, with less extensive surgical procedures considered for small, intrathyroidal tumors with favorable histological types.
  • Prophylactic central node dissection is controversial, but may be considered in cases with preoperative suspicion or intraoperative proof of lymph node metastases.

From the Research

Indications for Biopsy

  • Thyroid nodules with suspicious ultrasonography findings, such as hypoechogenicity, irregular margins, absence of halo, taller-than-wide shape, increased vascularity, and microcalcifications, should be considered for biopsy, regardless of cytological results 4
  • Nodules larger than 1 cm should be biopsied, especially if they have features suggestive of malignancy or if the patient has risk factors for thyroid cancer 5
  • The American Association of Clinical Endocrinologists guidelines recommend biopsying hypoechoic nodules with at least one additional suspicious feature, such as irregular margins, length greater than width, and microcalcifications 6

Ultrasound Patterns and Biopsy

  • The 2015 American Thyroid Association (ATA) guidelines provide a classification system for ultrasound patterns, which can help identify nodules with a high suspicion of malignancy 7
  • Nodules with very low or low suspicion of malignancy on ultrasound can be followed up with ultrasound, while those with intermediate or high suspicion should be evaluated with repeat fine-needle aspiration biopsy 7
  • The TIRADS classification system can help identify subcentimeter nodules that should be biopsied, with higher classifications (4A, 4B, 4C, and 5) indicating a higher risk of malignancy 8

Nodule Size and Biopsy

  • Nodules smaller than 1 cm can be biopsied if they have suspicious ultrasound features or if the patient has risk factors for thyroid cancer 5, 8
  • Subcentimeter nodules with TIRADS classifications of 4A, 4B, 4C, or 5 should be considered for biopsy due to the higher risk of malignancy 8

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.