What is the recommended workup and management for endometriosis?

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Last updated: November 3, 2025View editorial policy

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Workup for Endometriosis

Initial Clinical Assessment

The diagnosis of endometriosis is fundamentally clinical and does not require surgical confirmation before initiating empiric treatment. 1 Begin by identifying key symptom patterns and physical examination findings that strongly suggest endometriosis, as these alone can justify starting treatment.

Key Clinical Features to Document

  • Pain patterns: Dysmenorrhea, dyspareunia (deep penetration pain), dyschezia (painful bowel movements), dysuria, or chronic pelvic pain 1, 2
  • Infertility: Present in approximately 26-50% of patients with endometriosis 1, 2
  • Physical examination findings: Nodularity in the posterior fornix, fixed retroverted uterus, tender uterosacral ligaments, or visible lesions on speculum examination 1, 3

Important caveat: Normal physical examination does not exclude endometriosis—superficial peritoneal disease is poorly detected by examination and imaging. 1, 2

Imaging Algorithm

Transvaginal ultrasound (TVUS) is the mandatory first-line imaging modality. 1 However, standard TVUS protocols are insufficient for deep endometriosis detection.

Imaging Protocol Selection

  • Expanded protocol TVUS (with bowel preparation and systematic evaluation of deep compartments) demonstrates excellent performance for deep endometriosis, with sensitivity 82.5% and specificity 84.6% 1, 3
  • Add transabdominal ultrasound to widen the field of view for urinary tract and bowel involvement beyond the pelvis 1
  • MRI pelvis without IV contrast is equally appropriate as first-line imaging and is sufficient for detecting deep endometriosis 1
  • MRI with IV contrast is highly recommended when differentiating endometriomas from ovarian malignancies 1

Critical pitfall: Do not rely on standard TVUS alone for deep endometriosis—expanded protocols or MRI are needed. 1 Standard protocols miss significant disease.

When to Use Advanced Imaging

  • MRI should be obtained if TVUS is inconclusive, particularly when coexisting pathologies like leiomyomas are present (TVUS sensitivity drops to 33.3% in these cases) 1, 4
  • CT pelvis has no role in standard endometriosis diagnosis and should not be ordered 1

Laboratory Testing

Pregnancy Test

  • Check serum β-hCG to rule out pregnancy in all reproductive-age women with pelvic pain 5

Tumor Markers

  • CA-125 has no clinical utility for diagnosis 6
  • CA-125 may be helpful for monitoring clinical response in patients with confirmed extrauterine disease, but can be falsely elevated due to peritoneal inflammation/infection 6, 1
  • Do not order CA-125 for diagnostic purposes 6

Hormonal Assessment

  • Not routinely indicated for diagnosis 5
  • Consider FSH, LH, and estradiol only if amenorrhea is present or ovarian function assessment is needed for other clinical reasons 5

What This Workup Accomplishes

This imaging-first approach serves multiple critical functions beyond diagnosis:

  • Enables better surgical planning by mapping disease extent preoperatively 1
  • Reduces surgical morbidity by preventing incomplete surgeries that require reoperation 1
  • Identifies deep infiltrating disease that may require bowel or urologic surgery 6, 1
  • Detects endometriomas that influence fertility management decisions 1

When Surgical Diagnosis Is Needed

Laparoscopy for definitive diagnosis is NOT required before starting empiric hormonal treatment. 1, 2 Surgery should be reserved for:

  • Failure of first-line hormonal therapy 2
  • Contraindications to hormonal therapy 2
  • Suspected deep infiltrating disease requiring excision 6
  • Fertility concerns where surgical treatment may improve outcomes 7

Important consideration: Diagnostic delay averages 5-12 years after symptom onset, with most women consulting 3 or more clinicians before diagnosis. 2 Do not contribute to this delay by requiring surgical confirmation before treatment.

Associated Conditions to Screen For

  • Adenomyosis: Often coexists with endometriosis and requires MRI for diagnosis when ultrasound is equivocal (MRI sensitivity 78%, specificity 93%) 1, 4
  • Cardiovascular risk: Women with endometriosis have increased risk for stroke (HR 1.34,95% CI 1.10-1.62) 1
  • Ovarian cancer risk: Increased risk of clear cell, endometrioid, and low-grade serous ovarian cancers (0.7-1.6% lifetime risk) 3

Quality of Life Assessment

  • Document baseline quality of life using validated instruments such as the Endometriosis Health Profile-30 (EHP-30), EHP-5, or SF-36 3
  • This establishes baseline for monitoring treatment response 3

References

Guideline

Diagnosing Endometriosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Endometriosis: A Review.

JAMA, 2025

Research

Evaluation and management of endometriosis.

Climacteric : the journal of the International Menopause Society, 2023

Guideline

Adenomyosis Diagnosis and Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Management of Amenorrhea After Lupron Treatment for Endometriosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Clinical Management of Endometriosis.

Obstetrics and gynecology, 2018

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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