What does a positive Hepatobiliary Iminodiacetic Acid (HIDA) scan result indicate?

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What is a Positive HIDA Scan Result?

A positive HIDA scan result indicates non-visualization of the gallbladder due to cystic duct obstruction, which is diagnostic of acute cholecystitis. 1, 2

Primary Interpretation: Acute Cholecystitis

  • Non-filling of the gallbladder after radiotracer administration is the hallmark finding that defines a positive HIDA scan for acute cholecystitis 2
  • The radiotracer (technetium-99m labeled hepatobiliary iminodiacetic acid) is extracted by hepatocytes and normally clears through the biliary system, filling the gallbladder within 30-60 minutes 2
  • When the cystic duct is obstructed (typically by a gallstone), the radiotracer cannot enter the gallbladder, resulting in visualization of the liver and bile ducts but absence of gallbladder filling 2

Diagnostic Performance

HIDA scan has the highest sensitivity and specificity for acute cholecystitis compared to all other imaging modalities, with sensitivity of 84.2-89.3% versus 67.3% for ultrasound and 59.8% for CT (p=0.017) 1, 3

  • Specificity ranges from 66.8-79% 1
  • This superior diagnostic accuracy makes HIDA the gold standard when other imaging is equivocal 1

Alternative Interpretation: Chronic Acalculous Cholecystitis/Biliary Dyskinesia

A positive HIDA scan can also refer to gallbladder ejection fraction (GBEF) <35% after cholecystokinin (CCK) stimulation, which suggests chronic acalculous gallbladder disease or biliary dyskinesia 3, 4, 5, 2

  • This interpretation applies specifically to patients with chronic biliary-type pain without gallstones, not acute cholecystitis 3, 4
  • CCK-augmented HIDA scanning is NOT indicated for acute calculous cholecystitis where cystic duct obstruction is already present 3
  • Patients with GBEF <35% who undergo cholecystectomy have 15-fold greater odds of symptom improvement compared to medical management 5

Important Caveat About Ejection Fraction

Symptom reproduction during CCK infusion may be more clinically significant than the ejection fraction itself 4

  • In one study, all 42 patients (including 25 with normal GBEF >35%) who had symptom reproduction during CCK-HIDA and underwent cholecystectomy had resolution of symptoms at mean 18.7-month follow-up 4
  • Only 2.4% experienced symptom recurrence, suggesting that normal GBEF does not rule out biliary etiology when symptoms are reproduced 4

Clinical Context and Limitations

HIDA scan utilization in clinical practice is limited due to required resources, time, and need for patient fasting 1, 3

  • The test typically requires several hours to complete 3
  • Patients must fast for several hours beforehand 3
  • False-positive results can occur, and ERCP should be performed before surgical intervention if clinical suspicion is low 6

When HIDA is Indicated

HIDA scan should be considered when ultrasound findings are equivocal but clinical suspicion for acute cholecystitis remains high 3

  • Ultrasound remains the initial imaging modality of choice due to lower cost, portability, and lack of radiation 3
  • HIDA is reserved for selected patients depending on local expertise and availability 1
  • In the era of Tokyo Guidelines 2018, HIDA may be overutilized, as clinical criteria alone can establish diagnosis in many cases 7

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Nuclear medicine hepatobiliary imaging.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, 2010

Guideline

Diagnostic Approach for Gallstone Cholecystitis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

The cholecystokin provocation HIDA test: recreation of symptoms is superior to ejection fraction in predicting medium-term outcomes.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract, 2011

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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