What is the diagnostic workup for suspected Ankylosing Spondylitis (AS)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: March 28, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

From the Guidelines

The diagnostic workup for suspected Ankylosing Spondylitis (AS) should begin with a thorough clinical evaluation, including inflammatory back pain characteristics, followed by laboratory tests such as erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and HLA-B27 testing, and imaging studies, with magnetic resonance imaging (MRI) of the sacroiliac joints being preferred for early detection. The clinical evaluation should focus on characteristics such as morning stiffness lasting more than 30 minutes, improvement with exercise, pain at night with improvement upon arising, and onset before age 45 1. Laboratory tests, including ESR and CRP, may be elevated in active disease, and HLA-B27 testing is recommended as this genetic marker is present in a significant proportion of patients with AS, though it's not diagnostic on its own 1.

Imaging studies are crucial, starting with plain radiographs of the sacroiliac joints and spine to look for sacroiliitis and syndesmophytes. However, early disease may not show radiographic changes, so MRI of the sacroiliac joints is preferred for early detection as it can identify active inflammation before structural damage occurs 1. The Assessment of SpondyloArthritis international Society (ASAS) proposed classification criteria that apply to both patients in the early stage of the disease and those in the later stages, included under the umbrella term axial SpA 1.

Additional assessments should include evaluation for extra-articular manifestations such as uveitis, inflammatory bowel disease, and psoriasis. Referral to a rheumatologist is essential for confirming the diagnosis and initiating appropriate management. This comprehensive approach helps identify AS early, allowing for timely intervention to prevent disease progression and structural damage. The use of tumor necrosis factor inhibitors (TNFi) has greatly altered the approach to the treatment of AS, and recommendations are needed to help optimize care of these patients 1.

Key points to consider in the diagnostic workup include:

  • Clinical evaluation focusing on inflammatory back pain characteristics
  • Laboratory tests, including ESR, CRP, and HLA-B27 testing
  • Imaging studies, with MRI of the sacroiliac joints preferred for early detection
  • Evaluation for extra-articular manifestations
  • Referral to a rheumatologist for confirmation and management.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.